中国抗生素杂志
中國抗生素雜誌
중국항생소잡지
CHINESE JOURNAL OF ANTIBIOTICS
2007年
2期
104-107,S1
,共5页
凌保东%余娴%谢勇恩%雷军
凌保東%餘嫻%謝勇恩%雷軍
릉보동%여한%사용은%뢰군
阴沟肠杆菌%头孢菌素酶%ampC基因
陰溝腸桿菌%頭孢菌素酶%ampC基因
음구장간균%두포균소매%ampC기인
Enterobacter cloacae%AmpC β-lactamase%ampC gene
目的 研究我院临床分离阴沟肠杆菌的产AmpC酶耐药情况及ampC基因型.方法 收集临床分离的耐药阴沟肠杆菌15株;检测产AmpC酶和超广谱β-内酰胺酶(ESBLs)的菌株;测定MIC值;PCR扩增检测ampC基因及序列测定.结果 15株菌中8株菌(53.3%)产AmpC酶,3株菌(20.0%)产ESBLs.产AmpC酶的菌株除对亚胺培南全敏感外,对其它抗菌药不同程度耐药.ampC基因与阴沟肠杆菌ECLC074的ampC基因高度同源.结论 产AmpC酶是阴沟肠杆菌对β-内酰胺类抗生素耐药的主要机制之一.阴沟肠杆菌ECLC074 ampC基因是我院主要的阴沟肠杆菌ampC基因型.产AmpC酶的阴沟肠杆菌常呈多重耐药,亚胺培南是治疗此类菌所致感染的最有效药物.
目的 研究我院臨床分離陰溝腸桿菌的產AmpC酶耐藥情況及ampC基因型.方法 收集臨床分離的耐藥陰溝腸桿菌15株;檢測產AmpC酶和超廣譜β-內酰胺酶(ESBLs)的菌株;測定MIC值;PCR擴增檢測ampC基因及序列測定.結果 15株菌中8株菌(53.3%)產AmpC酶,3株菌(20.0%)產ESBLs.產AmpC酶的菌株除對亞胺培南全敏感外,對其它抗菌藥不同程度耐藥.ampC基因與陰溝腸桿菌ECLC074的ampC基因高度同源.結論 產AmpC酶是陰溝腸桿菌對β-內酰胺類抗生素耐藥的主要機製之一.陰溝腸桿菌ECLC074 ampC基因是我院主要的陰溝腸桿菌ampC基因型.產AmpC酶的陰溝腸桿菌常呈多重耐藥,亞胺培南是治療此類菌所緻感染的最有效藥物.
목적 연구아원림상분리음구장간균적산AmpC매내약정황급ampC기인형.방법 수집림상분리적내약음구장간균15주;검측산AmpC매화초엄보β-내선알매(ESBLs)적균주;측정MIC치;PCR확증검측ampC기인급서렬측정.결과 15주균중8주균(53.3%)산AmpC매,3주균(20.0%)산ESBLs.산AmpC매적균주제대아알배남전민감외,대기타항균약불동정도내약.ampC기인여음구장간균ECLC074적ampC기인고도동원.결론 산AmpC매시음구장간균대β-내선알류항생소내약적주요궤제지일.음구장간균ECLC074 ampC기인시아원주요적음구장간균ampC기인형.산AmpC매적음구장간균상정다중내약,아알배남시치료차류균소치감염적최유효약물.
Objective To determine the prevalence and genotype of chromosomal AmpC β-lactamases produced by clinical isolates of Enterobacter cloacae. Methods Fifteen randomly chosen piperacillin-resistant Enterobacter cloacae were isolated. AmpC β-lactamases and extended spectrum β-lactamases (ESBLs) were detected. MICs of antibacterials were determined. Their ampC genes were amplified by PCR and PCR products were sequenced. Results Eight isolates (53.3%) produced AmpC β-lactamases and other 3 strains (20.0%)produced ESBLs. AmpC β-lactamase producers were susceptible to imipenem, while they had different resistant rates to other antibacterials. The Enterobacter cloacae ECLC074 ampC gene and the ampC genes of these strains were highly homologous. The AmpC β-lactamases of 2 isolates had one amino acid residue mutation. Conclusions The production of AmpC β-lactamases in strains of Enterobacter cloacae is one of the main resistant mechanisms to β-lactam antibiotics. Enterobacter cloacae ECLC074 ampC gene is the main gene style of AmpC β-lactamases-producing Enterobacter cloacae. AmpC β-lactamase producers are multi-drug resistant and imipenem is the most effective antibiotic for treating infections caused by this kind of strains.
