河南大学学报（医学版）
河南大學學報（醫學版）
하남대학학보（의학판）
JOURNAL OF HENAN UNIVERSITY (MEDICAL SCIENCE)
2008年
3期
35-37
,共3页
贺光东%郅妙利%王二辉%康文艺
賀光東%郅妙利%王二輝%康文藝
하광동%질묘리%왕이휘%강문예
HPLC%QA%茜草根
HPLC%QA%茜草根
HPLC%QA%천초근
目的:测定陕西产茜草中1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量.方法:选用Purospher star RP-C18色谱柱(4.6 mm×250 mm, 5 μm, Merck公司);流动相为甲醇-水-四氢呋喃 (65∶34.7∶0.3),流速为1.0 ml/min,检测波长为276 nm,柱温为25℃,外标法定量分析.结果:该方法在0.020 μg～0.160 μg范围内线性关系良好,r＝0.9999,平均回收率101.4,RSD＝2.0% (n=6),测定结果为0.053%.结论:该法测定茜草中1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]含量灵敏、准确、重现性好.
目的:測定陝西產茜草中1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量.方法:選用Purospher star RP-C18色譜柱(4.6 mm×250 mm, 5 μm, Merck公司);流動相為甲醇-水-四氫呋喃 (65∶34.7∶0.3),流速為1.0 ml/min,檢測波長為276 nm,柱溫為25℃,外標法定量分析.結果:該方法在0.020 μg～0.160 μg範圍內線性關繫良好,r＝0.9999,平均迴收率101.4,RSD＝2.0% (n=6),測定結果為0.053%.結論:該法測定茜草中1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]含量靈敏、準確、重現性好.
목적:측정협서산천초중1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]적함량.방법:선용Purospher star RP-C18색보주(4.6 mm×250 mm, 5 μm, Merck공사);류동상위갑순-수-사경부남 (65∶34.7∶0.3),류속위1.0 ml/min,검측파장위276 nm,주온위25℃,외표법정량분석.결과:해방법재0.020 μg～0.160 μg범위내선성관계량호,r＝0.9999,평균회수솔101.4,RSD＝2.0% (n=6),측정결과위0.053%.결론:해법측정천초중1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]함량령민、준학、중현성호.
