CAJ | 학술논문

本文报道多元校正紫外光度法同时测定甲硝唑和维生素B6.首先在0.1 mol/L HCl溶液中对甲硝唑和维生素B6两组分混合溶液进行分光光度法测定,然后将所得的重叠光谱数据经计算机采集后,分别用化学计量学方法中的偏最小二乘法(PLS)和主成分回归法(PCR)进行处理,并用于药物样品的测定,获得了较好的定量分析结果.该法甲硝唑和维生素B6的线性范围分别为1.0～28.0 mg/L和1.0～28.0 mg/L,检出限分别为0.568 mg/L和0.364 mg/L.
본문보도다원교정자외광도법동시측정갑초서화유생소B6.수선재0.1 mol/L HCl용액중대갑초서화유생소B6량조분혼합용액진행분광광도법측정,연후장소득적중첩광보수거경계산궤채집후,분별용화학계량학방법중적편최소이승법(PLS)화주성분회귀법(PCR)진행처리,병용우약물양품적측정,획득료교호적정량분석결과.해법갑초서화유생소B6적선성범위분별위1.0～28.0 mg/L화1.0～28.0 mg/L,검출한분별위0.568 mg/L화0.364 mg/L.
Metronidazole and Vitamin B6 have overlapping spectra in UV range, and it is difficult to determine them individually. The chemometric multivariate calibration methods, such as partial least squares (PLS) and principal components regression (PCR) were applied to the simultaneous determination of metronidazole and Vitamin B6 in 0.1 mol/L hydrochloric acid by resolving the overlapping spectra. The linear ranges were 1.0 ～ 28.0 mg/L and 1.0 ～ 28.0 mg/L for metronidazole and vitamin B6,respectively. The limits of detection were 0.568 mg/L and 0.364 mg/L for metronidazole and vitamin B6, respectively. These methods were successfully applied to the analysis of samples with satisfied results.