国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2009年
16期
1-3
,共3页
鲍曼不动杆菌%最低抑菌浓度%主动外排
鮑曼不動桿菌%最低抑菌濃度%主動外排
포만불동간균%최저억균농도%주동외배
Acinetobacter baumannii%The minimum inhibitory concentration (MIC)%Active efflux
目的 比较碳酰氰间氯苯腙(CCCP)、利血平、奥美拉唑三种泵抑制剂对鲍曼不动杆菌外排泵筛选效果.选择适合的主动外排抑制剂.方法 应用琼脂稀释法检测环丙沙星对76株鲍曼不动杆菌的最低抑菌浓度(MIC),同时分别检测在加入主动外排抑制剂:CCCP、利血平、奥美拉唑后,MIC的变化情况,MIC降低4倍以上的菌株为外排泵阳性.结果 加入CCCP、利血平、奥美拉唑三种泵抑制剂后均可不同程度地减少菌体对环内沙星的外排作用,降低环丙沙星对鲍曼不动杆菌的MIC,三者的MIC50、MIC90分别为16 μg/ml、64 μg/ml,32 μg/ml、168 μg/ml,32 μg/ml、168 μg/ml,三者检出外排泵阳性率分别为26.3%、10.5%、9.2%.结论 CCCP、利血平、奥美拉唑均可不同程度地减少菌体对环丙沙星的外排作用,但CCCP更适合作为筛选鲍曼不动杆菌主动外排泵的抑制剂.
目的 比較碳酰氰間氯苯腙(CCCP)、利血平、奧美拉唑三種泵抑製劑對鮑曼不動桿菌外排泵篩選效果.選擇適閤的主動外排抑製劑.方法 應用瓊脂稀釋法檢測環丙沙星對76株鮑曼不動桿菌的最低抑菌濃度(MIC),同時分彆檢測在加入主動外排抑製劑:CCCP、利血平、奧美拉唑後,MIC的變化情況,MIC降低4倍以上的菌株為外排泵暘性.結果 加入CCCP、利血平、奧美拉唑三種泵抑製劑後均可不同程度地減少菌體對環內沙星的外排作用,降低環丙沙星對鮑曼不動桿菌的MIC,三者的MIC50、MIC90分彆為16 μg/ml、64 μg/ml,32 μg/ml、168 μg/ml,32 μg/ml、168 μg/ml,三者檢齣外排泵暘性率分彆為26.3%、10.5%、9.2%.結論 CCCP、利血平、奧美拉唑均可不同程度地減少菌體對環丙沙星的外排作用,但CCCP更適閤作為篩選鮑曼不動桿菌主動外排泵的抑製劑.
목적 비교탄선청간록분종(CCCP)、리혈평、오미랍서삼충빙억제제대포만불동간균외배빙사선효과.선택괄합적주동외배억제제.방법 응용경지희석법검측배병사성대76주포만불동간균적최저억균농도(MIC),동시분별검측재가입주동외배억제제:CCCP、리혈평、오미랍서후,MIC적변화정황,MIC강저4배이상적균주위외배빙양성.결과 가입CCCP、리혈평、오미랍서삼충빙억제제후균가불동정도지감소균체대배내사성적외배작용,강저배병사성대포만불동간균적MIC,삼자적MIC50、MIC90분별위16 μg/ml、64 μg/ml,32 μg/ml、168 μg/ml,32 μg/ml、168 μg/ml,삼자검출외배빙양성솔분별위26.3%、10.5%、9.2%.결론 CCCP、리혈평、오미랍서균가불동정도지감소균체대배병사성적외배작용,단CCCP경괄합작위사선포만불동간균주동외배빙적억제제.
Objective compare the screen effect of the Acinetobacter baumannii efflux pump by carbonyl cyanide m-chlorophenylhydrazone (CCCP),reserpine and omeprasole,in order to choose the suitable active efflux inhibitiors.Methods Test the minimum inhibitory concentration (MIC) of the cipmfloxacin on 76 strains of Acinetobacter baumanniis by agar dilution method.Meanwhile,the influence of active efflux inhibifiors CCCP,reserpine and omeprazole on MIC Were measured as well.The strains whose MIC decreased four times are positive efflux pumps.Results CCCP,reserpine and omeprazole have effect on the strains' efflux of ciprofloxacin in different degree and all decreased the MIC of ciporfloxacin on the Acinetobacter baumannii,with the MIC50 and MIC90 being 16μg/ml、64μg/ml,32μg/ml、168μg/ml,321μg/ml、168μg/ml.,The rate of positive efflux pumps are respectively 26.3%、10.5%、9.2%.Conclusions CCCP,reserpine and omeprazole can decrease the strains' efflux of ciporfloxacin in different degree,but CCCP is more suitable to act as an active eflux pump inhibitor to screen the Acinetobacter baumannii.
