国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
20期
1526-1529
,共4页
高元妹%夏旸%沈湘波%张平%赵海金%蔡绍曦
高元妹%夏旸%瀋湘波%張平%趙海金%蔡紹晞
고원매%하양%침상파%장평%조해금%채소희
屋尘螨%血管内皮生长因子%支气管上皮细胞通透性
屋塵螨%血管內皮生長因子%支氣管上皮細胞通透性
옥진만%혈관내피생장인자%지기관상피세포통투성
House dust mites%Vascular endothelial growth factor%Bronchial epithelial permeability
目的 探讨屋尘螨对支气管上皮细胞通透性的影响.方法 通过ELISA、荧光定量PCR检测屋尘螨刺激人的支气管上皮细胞后对血管内皮生长因子(VEGF)表达的影响,通过检测细胞的跨膜电阻了解屋尘螨对人的支气管上皮细胞通透性的影响.结果 屋尘螨可以上调人的支气管上皮细胞VEGF的表达,且呈明显的时间依赖性和浓度依赖性,并与支气管上皮细胞的通透性呈正相关,VEGF的单克隆抗体可以明显阻断这一效应.结论 屋尘螨通过上调VEGF的表达增加了对支气管上皮细胞的通透性.
目的 探討屋塵螨對支氣管上皮細胞通透性的影響.方法 通過ELISA、熒光定量PCR檢測屋塵螨刺激人的支氣管上皮細胞後對血管內皮生長因子(VEGF)錶達的影響,通過檢測細胞的跨膜電阻瞭解屋塵螨對人的支氣管上皮細胞通透性的影響.結果 屋塵螨可以上調人的支氣管上皮細胞VEGF的錶達,且呈明顯的時間依賴性和濃度依賴性,併與支氣管上皮細胞的通透性呈正相關,VEGF的單剋隆抗體可以明顯阻斷這一效應.結論 屋塵螨通過上調VEGF的錶達增加瞭對支氣管上皮細胞的通透性.
목적 탐토옥진만대지기관상피세포통투성적영향.방법 통과ELISA、형광정량PCR검측옥진만자격인적지기관상피세포후대혈관내피생장인자(VEGF)표체적영향,통과검측세포적과막전조료해옥진만대인적지기관상피세포통투성적영향.결과 옥진만가이상조인적지기관상피세포VEGF적표체,차정명현적시간의뢰성화농도의뢰성,병여지기관상피세포적통투성정정상관,VEGF적단극륭항체가이명현조단저일효응.결론 옥진만통과상조VEGF적표체증가료대지기관상피세포적통투성.
Objective To investigate whether house dust mites (HDM) increase pemeability in bronchial epithelial permeability.Methods We estimated HDM induced VEGF release in NHBE by using an ELISA and VEGF mRNA expression by using an RT-PCR reaction.The influence of HDM on NHBE barrier function was estimated by measuring electrical resistance with an electric cell substrate impedancesensing system.Results Our results demonstrate that HDM induces VEGF release in NHBE in a timedependent and concentration-dependent manner.HDM decreased electrical resistance across NHBE monolayers.Neutralizing antibodies to VEGF significantly inhibited the decrease in NHBE electrical resistance caused by HDM.Conclusions HDM increases permeability of bronchial epithelial monolayer via VEGF induction.
