中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2007年
5期
290-293
,共4页
葛林虎%彭思达%王春燕%谭获
葛林虎%彭思達%王春燕%譚穫
갈림호%팽사체%왕춘연%담획
黑色素瘤%基因表达%碱性成纤维生长因子%细胞载体%微血管密度
黑色素瘤%基因錶達%堿性成纖維生長因子%細胞載體%微血管密度
흑색소류%기인표체%감성성섬유생장인자%세포재체%미혈관밀도
Melanoma%Gene expression%Basic fibroblast growth factor%Vector%Microvessel density
目的 探讨转染同源盒第7基因(HOXB7)siRNA质粒表达载体对人恶性黑色素瘤细胞株A375在裸鼠体内生长的影响.方法 裸鼠皮下接种人恶性黑色素瘤A375细胞,对2周后形成的瘤块进行分组干预.随机分为生理盐水对照组、阴性质粒组、HOXB7质粒组,观察转染后各组裸鼠移植瘤的生长情况;免疫组化法比较瘤体内微血管密度(MVD).结果 HOXB7质粒组的裸鼠移植瘤块生长慢、体积明显小于生理盐水对照组[(0.134±0.039)cm3比(1.006±0.235)cm3,P＜0.05],而阴性质粒组瘤块体积大小与生理盐水对照组无统计学差异[(0.929±0.157)cm3比(1.006±0.235)cm3,P＞0.05].HOXB7质粒组裸鼠体内肿瘤MVD低于生理盐水对照组[(2.8±1.9)比(19.9±5.6),P＜0.05],阴性质粒组与生理盐水对照组无统计学差异[(18.1±5.5)比(19.9±5.6),P＞0.05].结论 针对HOXB7 siRNA质粒表达载体可有效抑制A375细胞裸鼠体内肿瘤生长和瘤内血管生成.
目的 探討轉染同源盒第7基因(HOXB7)siRNA質粒錶達載體對人噁性黑色素瘤細胞株A375在裸鼠體內生長的影響.方法 裸鼠皮下接種人噁性黑色素瘤A375細胞,對2週後形成的瘤塊進行分組榦預.隨機分為生理鹽水對照組、陰性質粒組、HOXB7質粒組,觀察轉染後各組裸鼠移植瘤的生長情況;免疫組化法比較瘤體內微血管密度(MVD).結果 HOXB7質粒組的裸鼠移植瘤塊生長慢、體積明顯小于生理鹽水對照組[(0.134±0.039)cm3比(1.006±0.235)cm3,P＜0.05],而陰性質粒組瘤塊體積大小與生理鹽水對照組無統計學差異[(0.929±0.157)cm3比(1.006±0.235)cm3,P＞0.05].HOXB7質粒組裸鼠體內腫瘤MVD低于生理鹽水對照組[(2.8±1.9)比(19.9±5.6),P＜0.05],陰性質粒組與生理鹽水對照組無統計學差異[(18.1±5.5)比(19.9±5.6),P＞0.05].結論 針對HOXB7 siRNA質粒錶達載體可有效抑製A375細胞裸鼠體內腫瘤生長和瘤內血管生成.
목적 탐토전염동원합제7기인(HOXB7)siRNA질립표체재체대인악성흑색소류세포주A375재라서체내생장적영향.방법 라서피하접충인악성흑색소류A375세포,대2주후형성적류괴진행분조간예.수궤분위생리염수대조조、음성질립조、HOXB7질립조,관찰전염후각조라서이식류적생장정황;면역조화법비교류체내미혈관밀도(MVD).결과 HOXB7질립조적라서이식류괴생장만、체적명현소우생리염수대조조[(0.134±0.039)cm3비(1.006±0.235)cm3,P＜0.05],이음성질립조류괴체적대소여생리염수대조조무통계학차이[(0.929±0.157)cm3비(1.006±0.235)cm3,P＞0.05].HOXB7질립조라서체내종류MVD저우생리염수대조조[(2.8±1.9)비(19.9±5.6),P＜0.05],음성질립조여생리염수대조조무통계학차이[(18.1±5.5)비(19.9±5.6),P＞0.05].결론 침대HOXB7 siRNA질립표체재체가유효억제A375세포라서체내종류생장화류내혈관생성.
Objective To investigate the effect of HOXB7 siRNA vector on the growth of human malignant melanoma in nude mice. Methods A375 cells were inoculated subcutaneously to nude mouse.When the tumor formed after 2weeks, mice were divided into three groups randomly and equally:the normal saline(NS)group,the negative control group injected with empty plasmid and the group which injected with the siRNA expressing vector targeting HOXB7 gene. The growth of xenograft tumor was observed, and the microvessel density(MVD) in tumor was estimated by immunohistochemistry stain. Results The growth speed and size of xenogeaft tumor interfered by the HOXB7 siRNA expressing vector decreased significantly,compared with those in the NS group [(0.134±0.039) cm2 vs (1. 006±0.235 ) cm3, P＜0.05 ]. There was no significant difference between the NS group and the negative group [(1. 006±0. 235 )cm3 vs (0. 929±0. 157)cm3, P＞0. 05]. The MVD of tumors in NS group, negative group and siRNA expression vector group were 19.9±5.6,18. 1±5.5 and 2.8±1.9, respectively. Conclusion Sequence-specific siRNA expression vector targeting HOXB7 gene can inhibit the tumor growth and angiogenesis in vivo.
