中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
1期
45-47
,共3页
许长琼%王伟%王怀宁%刘德红%冯晓玲
許長瓊%王偉%王懷寧%劉德紅%馮曉玲
허장경%왕위%왕부저%류덕홍%풍효령
抗原,CD14%内毒素类%感染%肿瘤坏死因子α%白细胞介素6%白细胞介素10%预后
抗原,CD14%內毒素類%感染%腫瘤壞死因子α%白細胞介素6%白細胞介素10%預後
항원,CD14%내독소류%감염%종류배사인자α%백세포개소6%백세포개소10%예후
Antigens,CD14%Endotoxins%Infection%Tumor necrosis factor-alpha%Interleukin-6%Interleukin-10%Prognosis
目的 探讨内毒素受体CD14-159C/T基因多态性与严重感染患者预后及炎症介质的关系.方法 以2007年2月～7月收治的42例术后严重感染患者为对象,提取全血基因组DNA,应用限制性长度片段多态性(RFLP)方法检测CD14-159C/T基因多态性,观察CD14基因多态性分布与严重感染患者预后及细胞因子肿瘤坏死因子-α(TNF-α)、白介素一6(IL-6)、白介素-10(IL-10)的关系.结果 -159TT基因型患者死亡率(69.2%)明显高于-159CT(23.1%)及-159CC患者(7.7%)(P<0.05).-159TT纯合子TNF-α、IL-6的表达明显高于基因-159CT(P<0.05)及-159CC(P<0.05),而IL-10的表达明显低于后两者(P<0.05).结论 CD14-159TT基因型可能对严重感染患者的预后有一定影响,其机制可能与增加促炎因子TNF-α、IL-6表达及降低抑炎因子IL-10有关.
目的 探討內毒素受體CD14-159C/T基因多態性與嚴重感染患者預後及炎癥介質的關繫.方法 以2007年2月～7月收治的42例術後嚴重感染患者為對象,提取全血基因組DNA,應用限製性長度片段多態性(RFLP)方法檢測CD14-159C/T基因多態性,觀察CD14基因多態性分佈與嚴重感染患者預後及細胞因子腫瘤壞死因子-α(TNF-α)、白介素一6(IL-6)、白介素-10(IL-10)的關繫.結果 -159TT基因型患者死亡率(69.2%)明顯高于-159CT(23.1%)及-159CC患者(7.7%)(P<0.05).-159TT純閤子TNF-α、IL-6的錶達明顯高于基因-159CT(P<0.05)及-159CC(P<0.05),而IL-10的錶達明顯低于後兩者(P<0.05).結論 CD14-159TT基因型可能對嚴重感染患者的預後有一定影響,其機製可能與增加促炎因子TNF-α、IL-6錶達及降低抑炎因子IL-10有關.
목적 탐토내독소수체CD14-159C/T기인다태성여엄중감염환자예후급염증개질적관계.방법 이2007년2월～7월수치적42례술후엄중감염환자위대상,제취전혈기인조DNA,응용한제성장도편단다태성(RFLP)방법검측CD14-159C/T기인다태성,관찰CD14기인다태성분포여엄중감염환자예후급세포인자종류배사인자-α(TNF-α)、백개소일6(IL-6)、백개소-10(IL-10)적관계.결과 -159TT기인형환자사망솔(69.2%)명현고우-159CT(23.1%)급-159CC환자(7.7%)(P<0.05).-159TT순합자TNF-α、IL-6적표체명현고우기인-159CT(P<0.05)급-159CC(P<0.05),이IL-10적표체명현저우후량자(P<0.05).결론 CD14-159TT기인형가능대엄중감염환자적예후유일정영향,기궤제가능여증가촉염인자TNF-α、IL-6표체급강저억염인자IL-10유관.
Objective To investigate the relation of a lipopolysaecharide receptor CDl4C-159T gene polymorphism to severe sepsis outcome and cytokines in postoperative severe sepsis.Methods A prospective,consecutive entry study was made among 42 postoperative sevel'e sepsis admitted in neurosurgeon department,Second People～Hospital ShenZhen between Feb.2007 to Jul.2007.The genomie DNA of peripheral blood nucleated cells WaS extracted.CDl4-159C/T gene polymorphism patients was detected by restrictive fragment length polymorphism(RFLP)analysis.The relationship between genotypes and the production of eytokines TNF-α,IL-6,IL-10 and mortality rate of severe sepsis were evaluated.Results The mortality of sepsis in patients with CDl4 homozygous for the T allete(Tr)(69.2％)was significanfly higher than those with genotype CT(23.1％)and C allete(CC)(7.7％).In addition,TNF-a and IL-6 production ofTI'homozygore were markedly higher than those of the TC and CC genetypes.while IL-10 production were the lowest of the three(P<0.05).Conclusion The single base pair pelymorphism at position-159 in the CD14 gene promoter might influence the outcome of severe sepsis and may be related to production of pro-inflammatory cytokines TNF-α,IL-6,and decrease the anti-inflanunatiry factor IL-10.
