CAJ | 학술논문

目的探讨吗啡诱发条件性位置偏爱激活时大鼠伏隔核、前额叶皮层磷酸化cAMP反应元件结合蛋白(phospho-cAMP response element binding protein,p-CREB)和c-Fos表达的变化.方法以剂量递增法连续皮下注射(SC)吗啡6d建立大鼠条件性位置偏爱(conditioned place preference,CPP)模型,第7天用生理盐水替代吗啡训练大鼠10d,使形成的cPP逐渐消退,单次SC 4mg·kg-1吗啡激发已消退的CPP.采用免疫组化技术测定吗啡激发CPP重现时大鼠伏隔核、前额叶皮层p-CREB和c-Fos的变化.结果 (1)吗啡可使大鼠产生CPP效应,吗啡4mg·kg-1可使已消失的CPP效应激活;(2)与对照组相比吗啡诱发的CPP激活时大鼠伏隔核、前额叶皮层p-CREB(161.18±5.37,179.75±7.68)和c-Fos(176.63±8.42,185.11±5.61)的表达增加,与NS组比较差异有显著性(P＜0.05).结论伏隔核、前额叶皮层p-CREB和c-Fos蛋白表达参与了吗啡的CPP重现.
목적 탐토마배유발조건성위치편애격활시대서복격핵、전액협피층린산화cAMP반응원건결합단백(phospho-cAMP response element binding protein,p-CREB)화c-Fos표체적변화.방법 이제량체증법련속피하주사(SC)마배6d건립대서조건성위치편애(conditioned place preference,CPP)모형,제7천용생리염수체대마배훈련대서10d,사형성적cPP축점소퇴,단차SC 4mg·kg-1마배격발이소퇴적CPP.채용면역조화기술측정마배격발CPP중현시대서복격핵、전액협피층p-CREB화c-Fos적변화.결과 (1)마배가사대서산생CPP효응,마배4mg·kg-1가사이소실적CPP효응격활;(2)여대조조상비마배유발적CPP격활시대서복격핵、전액협피층p-CREB(161.18±5.37,179.75±7.68)화c-Fos(176.63±8.42,185.11±5.61)적표체증가,여NS조비교차이유현저성(P＜0.05).결론 복격핵、전액협피층p-CREB화c-Fos단백표체삼여료마배적CPP중현.
Objective To detect the effect of morphine-reactived conditioned place preference(CPP)on phospho-cAMP response element binding protein(p-CREB)and c-Fos expression in rat nucleus accumbens and prefrontal codex. Methods Morphine was administered by subcutaneous injection at gradually increasing dose tered saline instead of morphine for 10 days to induce CPP extinction. The rats were given a single priming injection immunohistochemistry method in the phase of reoccurrence of CPP. Results(1)Increasing dose of morphine days drug free period. (2)Compared to saline control, morphine-reactivated CPP elevated the expression of PCREB and c-Fos in nucleus accumbens and prefrontal codex in rats(P＜0. 05). Conclusion p-CREB and c-Fos expression in nucleus accumbens, prefrontal cortex may be involved in the mechanisms of reoccurrence of CPP.