CAJ | 학술논문

目的评价安络化纤丸联合干扰素γ治疗鼠血吸虫肝纤维化的效应机制及其对肝色素沉积的影响.方法将30只昆明小鼠分为健康对照组、感染对照组及干扰素γ+安络化纤丸治疗组.采用日本血吸虫尾蚴(40条/只)攻击感染小鼠,建立血吸虫性肝纤维化模型,连续干预8周,观察肝色素沉积及血吸虫卵肉芽肿改变;免疫组织化学检测肝组织Ⅰ型和Ⅲ型胶原的表达;荧光定量法检测肝组织TGF-β1 mRNA、组织病理学评价及电子计算机图像定量分析.对数据进行正态性检验、方差齐性检验及单因素方差分析.结果肝色素沉积百分比与TGF-β1 mRNA量呈相关性,相关系数=0.8;安络化纤丸联合干扰素γ治疗后明显减轻鼠血吸虫肝组织纤维化、减少色素沉着、使虫卵肉芽肿变小、下调Ⅰ及Ⅲ型胶原的表达及减少TGF-β1 mRNA量表达,与感染对照组比较,差异均有统计学意义(P<0.05).结论肝色素沉积量与TGF-β1 mRNA量有一定相关性,安络化纤丸联合干扰素γ明显减轻鼠血吸虫性肝纤维化.下调Ⅰ型及Ⅲ型胶原及TGF-β1 mRNA表达、减少色素沉积是其作用机制之一.
목적 평개안락화섬환연합간우소γ치료서혈흡충간섬유화적효응궤제급기대간색소침적적영향.방법 장30지곤명소서분위건강대조조、감염대조조급간우소γ+안락화섬환치료조.채용일본혈흡충미유(40조/지)공격감염소서,건립혈흡충성간섬유화모형,련속간예8주,관찰간색소침적급혈흡충란육아종개변;면역조직화학검측간조직Ⅰ형화Ⅲ형효원적표체;형광정량법검측간조직TGF-β1 mRNA、조직병이학평개급전자계산궤도상정량분석.대수거진행정태성검험、방차제성검험급단인소방차분석.결과 간색소침적백분비여TGF-β1 mRNA량정상관성,상관계수=0.8;안락화섬환연합간우소γ치료후명현감경서혈흡충간조직섬유화、감소색소침착、사충란육아종변소、하조Ⅰ급Ⅲ형효원적표체급감소TGF-β1 mRNA량표체,여감염대조조비교,차이균유통계학의의(P<0.05).결론 간색소침적량여TGF-β1 mRNA량유일정상관성,안락화섬환연합간우소γ명현감경서혈흡충성간섬유화.하조Ⅰ형급Ⅲ형효원급TGF-β1 mRNA표체、감소색소침적시기작용궤제지일.
Objective To evaluate efficacy and mechanism of Anluohuaxian pilule combined with interferon-γ in the treatment of schistosomal liver fibrosis. To preliminarily study on the relationship of pigment deposition in liver and schistosomal liver fibrosis. Methods Thirty Kunming mice were divided into the normal control group, the infection control group and the combination of Anluohuaxian pilule and Interferon-γ treated group. Schistosomal liver fibrosis model was established by infection with 40 Schistosoma japonicum cercariae. The treated group was treated by combination of Anluohuaxian pilule and Interferon-γ for 8 weeks. The changes of pigment deposition and hepatic egg granuloma in Schistosoma japonicum infected mice were observed. Expressions of collagen Ⅰ and Ⅲ were detected by immunohistochemistry. Transforming growth factor (TGF)-β1 was detected by fluorescent polymerase chain reaetion(PCR). Histopathology and computer image analysis were applied to evaluate the change in the liver tissues. Results The amount of pigment deposition in liver was related to the expression of TGF-β1 mRNA (correlation coefficient = 0. 8). Compared to the infection control group, combination of Anluohuaxian pilule and Interferon-γ can lessen hepatic fibrosis(P<0.05). The combination therapy can also make pigment deposition less and hepatic granuloma smaller than the infection control group(P<0. 05). Conclusions Pigment deposition in liver is related to the expression of TGF-β 1. Combination of Anluohuaxian pilule and Interferon-γ can lessen hepatic fibrosis in mice infected with Schistosoma japonicum. It's one mechanism to of the combination therapy down-regulate the expression of collagen Ⅰ, Ⅲ and TGF-β 1.