中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2011年
2期
85-88
,共4页
李磊%陈杰%卢朝辉%于双妮%罗玉凤%赵武干%马怡晖%贾从伟
李磊%陳傑%盧朝輝%于雙妮%囉玉鳳%趙武榦%馬怡暉%賈從偉
리뢰%진걸%로조휘%우쌍니%라옥봉%조무간%마이휘%가종위
乳腺肿瘤%受体,趋化因子%肿瘤转移%CXCR3
乳腺腫瘤%受體,趨化因子%腫瘤轉移%CXCR3
유선종류%수체,추화인자%종류전이%CXCR3
Breast neoplasms%Receptors,chemokine%Neoplasm metastasis%CXCR3
目的 研究趋化因子受体CXCR3在乳腺癌中的表达,分析其与乳腺癌患者相关临床病理指标之间的关系.方法 应用逆转录聚合酶链反应(RT-PCR)和即时荧光定量PCR检测18例乳腺癌及癌旁正常组织中CXCR3 mRNA的表达水平;应用免疫组织化学SP法检测CXCR3在80例乳腺癌、20例乳腺纤维腺瘤和15例正常乳腺组织中的表达.结果 (1)RT-PCR和即时荧光定量PCR显示,18例乳腺癌组织CXCR3 mRNA的表达水平明显高于癌旁正常组织(P＜0.05).(2)免疫组织化学检测结果显示,CXCR3在乳腺癌组织中的表达率显著高于其在乳腺纤维腺瘤和正常乳腺组织中的表达(P＜0.05);乳腺癌组织中淋巴结转移阳性组CXCR3的表达率明显高于淋巴结转移阴性组(P＜0.05);乳腺癌组织中CXCR3的表达与患者淋巴结转移累及数目(P=0.000)、肿瘤大小(P=0.027)和pTNM分期(P=0.004)均呈显著正相关(P＜0.05).结论 趋化因子受体CXCR3在乳腺癌中高表达,提示其可能是参与乳腺癌病程进展的重要分子;CXCR3的表达与多项临床病理指标,尤其是淋巴结转移密切相关,提示其可作为预测乳腺癌淋巴结转移及预后的指标.
目的 研究趨化因子受體CXCR3在乳腺癌中的錶達,分析其與乳腺癌患者相關臨床病理指標之間的關繫.方法 應用逆轉錄聚閤酶鏈反應(RT-PCR)和即時熒光定量PCR檢測18例乳腺癌及癌徬正常組織中CXCR3 mRNA的錶達水平;應用免疫組織化學SP法檢測CXCR3在80例乳腺癌、20例乳腺纖維腺瘤和15例正常乳腺組織中的錶達.結果 (1)RT-PCR和即時熒光定量PCR顯示,18例乳腺癌組織CXCR3 mRNA的錶達水平明顯高于癌徬正常組織(P＜0.05).(2)免疫組織化學檢測結果顯示,CXCR3在乳腺癌組織中的錶達率顯著高于其在乳腺纖維腺瘤和正常乳腺組織中的錶達(P＜0.05);乳腺癌組織中淋巴結轉移暘性組CXCR3的錶達率明顯高于淋巴結轉移陰性組(P＜0.05);乳腺癌組織中CXCR3的錶達與患者淋巴結轉移纍及數目(P=0.000)、腫瘤大小(P=0.027)和pTNM分期(P=0.004)均呈顯著正相關(P＜0.05).結論 趨化因子受體CXCR3在乳腺癌中高錶達,提示其可能是參與乳腺癌病程進展的重要分子;CXCR3的錶達與多項臨床病理指標,尤其是淋巴結轉移密切相關,提示其可作為預測乳腺癌淋巴結轉移及預後的指標.
목적 연구추화인자수체CXCR3재유선암중적표체,분석기여유선암환자상관림상병리지표지간적관계.방법 응용역전록취합매련반응(RT-PCR)화즉시형광정량PCR검측18례유선암급암방정상조직중CXCR3 mRNA적표체수평;응용면역조직화학SP법검측CXCR3재80례유선암、20례유선섬유선류화15례정상유선조직중적표체.결과 (1)RT-PCR화즉시형광정량PCR현시,18례유선암조직CXCR3 mRNA적표체수평명현고우암방정상조직(P＜0.05).(2)면역조직화학검측결과현시,CXCR3재유선암조직중적표체솔현저고우기재유선섬유선류화정상유선조직중적표체(P＜0.05);유선암조직중림파결전이양성조CXCR3적표체솔명현고우림파결전이음성조(P＜0.05);유선암조직중CXCR3적표체여환자림파결전이루급수목(P=0.000)、종류대소(P=0.027)화pTNM분기(P=0.004)균정현저정상관(P＜0.05).결론 추화인자수체CXCR3재유선암중고표체,제시기가능시삼여유선암병정진전적중요분자;CXCR3적표체여다항림상병리지표,우기시림파결전이밀절상관,제시기가작위예측유선암림파결전이급예후적지표.
Objective To investigate the expression of CXCR3 and its association with clinicopathologic features in breast carcinoma. Methods The expression level of CXCR3 in 18 samples of breast cancer and corresponding normal tissues was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time RT-PCR analysis, Immunohistochemistry was carried out to examine the expression of CXCR3 in 80 breast cancers, 20 fibroadenomas and 15 normal breast tissues. Results ( 1 ) RT-PCR and real-time RT-PCR analysis showed a higher level of CXCR3 in breast cancer tissues than that in the corresponding normal breast tissues ( P ＜0. 05 ). ( 2 ) Immunohistochemistry analysis showed that the positive rate of CXCR3 in breast cancer tissues was significantly higher than that in fibroadenomas and the normal breast tissues( P ＜ 0. 05 ). The expression level of CXCR3 in the lymph node-positive group was higher than that in the lymph node-negative group ( P ＜ 0. 05 ). The expression of CXCR3 was positively correlated with the number of lymph nodes involved by metastasis, tumor size and pTNM tumor stage (P＜0. 05). Conclusions Chemokine receptor CXCR3 was up-regulated in breast cancer, and was associated with the progression of breast cancer. CXCR3 might be a novel molecular marker to predict lymph node metastasis and prognosis of breast cancer.
