CAJ | 학술논문

目的了解北京基因型结核分枝杆菌在天津市人群中的分布、流行特征及其与耐药表型的关系.方法收集2008年1月至2009年6月天津市结核病控制中心和10个区(县)结核病防治机构肺结核患者中初次分离培养的结核分枝杆菌656株及患者管理信息、临床信息和实验室信息.菌株对利福平、异烟肼、链霉素和乙胺丁醇的药物敏感性试验采用比例法.应用多重PCR法鉴定北京和非北京基因型菌株,并分析北京基因型菌株与耐药表型的关系.结果本研究中菌株的总耐药率为26.98%,耐多药率为6.25%.北京基因型菌株600株(91.46%),非北京基因型56株(8.53%),其分布与天津市户籍(x2=4.26,P=0.039)有关,未见与耐药表型间有明显相关性.结论北京基因型菌株是天津市的主要流行株,感染北京基因型结核分枝杆菌的天津市户籍患者的比例明显高于本地区流动人口患者,但尚未发现北京和非北京基因型菌株之间在耐药方面有明显的差异.
목적 료해북경기인형결핵분지간균재천진시인군중적분포、류행특정급기여내약표형적관계.방법 수집2008년1월지2009년6월천진시결핵병공제중심화10개구(현)결핵병방치궤구폐결핵환자중초차분리배양적결핵분지간균656주급환자관리신식、림상신식화실험실신식.균주대리복평、이연정、련매소화을알정순적약물민감성시험채용비례법.응용다중PCR법감정북경화비북경기인형균주,병분석북경기인형균주여내약표형적관계.결과 본연구중균주적총내약솔위26.98%,내다약솔위6.25%.북경기인형균주600주(91.46%),비북경기인형56주(8.53%),기분포여천진시호적(x2=4.26,P=0.039)유관,미견여내약표형간유명현상관성.결론 북경기인형균주시천진시적주요류행주,감염북경기인형결핵분지간균적천진시호적환자적비례명현고우본지구류동인구환자,단상미발현북경화비북경기인형균주지간재내약방면유명현적차이.
Objective To explore the distribution and characteristics on genotype of Mycobacterium tuberculosis and the relationship between Beijing genotype and drug-resistant phenotypes in Tianjin city. Methods 656 clinical strains were collected from Tianjin Center for Tuberculosis Control and ten other Tuberculosis Institute in Tianjin from January 2008 to June 2009.Information regarding administration, clinical as well as laboratory findings of patients were collected.Proportion method was adopted to detect the susceptibility on four anti-tuberculosis drugs, namely streptomycin (SM), isoniazid (INH), rifampicin (RFP) and ehambutol (EMB). Both Beijing and non-Beijing genotypes were differentiated by multiplex PCR. The relationship between Beijing genotype and drug-resistant phenotypes was analyzed. Results In this study, the overall resistance rate of MTB was 26.98%, with multidrug-resistant rate was 6.25%. Among 656 MTB strains, 600isolates (91.46% ) belonged to Beijing genotype. There was significant difference between Beijing and non-Beijing genotype (x2=4.26, P=0.039) among the Tianjin household registered population.Concerning the drug resistance, there was no significant difference between the two groups.Conclusion Beijing genotype strains were the predominant one in Tianjin. The proportion of people infected with the Beijing genotype strains in Tianjin household registration of patients was significantly higher than the proportion of patients in the floating population in the same region.Results from the statistical analysis did not reveal any statistically significant association between Beijing genotype and drug resistance.