中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
3期
182-185
,共4页
李亮萍%王倩%潘彦伶%张怡慧%魏佳优%陈翌华%高天明
李亮萍%王倩%潘彥伶%張怡慧%魏佳優%陳翌華%高天明
리량평%왕천%반언령%장이혜%위가우%진익화%고천명
C57BL/6J小鼠%NIH Swiss小鼠%抑郁%行为症状%模型,动物
C57BL/6J小鼠%NIH Swiss小鼠%抑鬱%行為癥狀%模型,動物
C57BL/6J소서%NIH Swiss소서%억욱%행위증상%모형,동물
C57BL/6J mice%NIH Swiss mice%Depression%Behavioral symptoms%Models,animal
目的 探讨两种不同遗传背景的小鼠C57BL/6J及NIH Swiss在抑郁模型中的行为学差异,为抗抑郁药物研究的实验动物选择提供参考,为研究遗传学因素在抑郁症发病过程中的作用提供有用的动物模型.方法 C57BL/6J、NIH Swiss小鼠各14只,利用强迫游泳实验和悬尾实验检测两种小鼠在应激条件下的抑郁样行为表现；利用旷场实验检测两种小鼠的自主运动能力.结果 强迫游泳实验中,C57BL/6J小鼠的不动时间明显长于NIH Swiss小鼠[(133.198±5.749)s比(80.265±10.939)s,P=0.000].悬尾实验中,C57BL/6J小鼠的不动时间高于NIH Swiss小鼠[(151.315±12.161)s比(95.107±14.649)s,P=0.007].而在旷场实验中,两种品系的小鼠运动总路程、站立次数、运动时间、边缘区运动路程、边缘区运动时间、中央区运动路程、中央区运动时间差异均无统计学意义.结论 C57BL/6J小鼠在急性应激的情况下更容易表现出抑郁样行为,而NIH Swiss小鼠则表现出一定的抑郁抗性,两种不同品系小鼠的不同行为学表现可能由于遗传因素导致.
目的 探討兩種不同遺傳揹景的小鼠C57BL/6J及NIH Swiss在抑鬱模型中的行為學差異,為抗抑鬱藥物研究的實驗動物選擇提供參攷,為研究遺傳學因素在抑鬱癥髮病過程中的作用提供有用的動物模型.方法 C57BL/6J、NIH Swiss小鼠各14隻,利用彊迫遊泳實驗和懸尾實驗檢測兩種小鼠在應激條件下的抑鬱樣行為錶現；利用曠場實驗檢測兩種小鼠的自主運動能力.結果 彊迫遊泳實驗中,C57BL/6J小鼠的不動時間明顯長于NIH Swiss小鼠[(133.198±5.749)s比(80.265±10.939)s,P=0.000].懸尾實驗中,C57BL/6J小鼠的不動時間高于NIH Swiss小鼠[(151.315±12.161)s比(95.107±14.649)s,P=0.007].而在曠場實驗中,兩種品繫的小鼠運動總路程、站立次數、運動時間、邊緣區運動路程、邊緣區運動時間、中央區運動路程、中央區運動時間差異均無統計學意義.結論 C57BL/6J小鼠在急性應激的情況下更容易錶現齣抑鬱樣行為,而NIH Swiss小鼠則錶現齣一定的抑鬱抗性,兩種不同品繫小鼠的不同行為學錶現可能由于遺傳因素導緻.
목적 탐토량충불동유전배경적소서C57BL/6J급NIH Swiss재억욱모형중적행위학차이,위항억욱약물연구적실험동물선택제공삼고,위연구유전학인소재억욱증발병과정중적작용제공유용적동물모형.방법 C57BL/6J、NIH Swiss소서각14지,이용강박유영실험화현미실험검측량충소서재응격조건하적억욱양행위표현；이용광장실험검측량충소서적자주운동능력.결과 강박유영실험중,C57BL/6J소서적불동시간명현장우NIH Swiss소서[(133.198±5.749)s비(80.265±10.939)s,P=0.000].현미실험중,C57BL/6J소서적불동시간고우NIH Swiss소서[(151.315±12.161)s비(95.107±14.649)s,P=0.007].이재광장실험중,량충품계적소서운동총로정、참립차수、운동시간、변연구운동로정、변연구운동시간、중앙구운동로정、중앙구운동시간차이균무통계학의의.결론 C57BL/6J소서재급성응격적정황하경용역표현출억욱양행위,이NIH Swiss소서칙표현출일정적억욱항성,량충불동품계소서적불동행위학표현가능유우유전인소도치.
Objective To investigate behavioral differences in models with depression between C57BL/6J and NIH Swiss mice with different genetic backgrounds,to provide references for selection of examination animals in anti- depression drug researches,and to provide the useful animal models for invcstigating the effects of genetic factors on occurrence of depression.Methods Behavioral manifestations of depression in C57BI/6J and NIH Swiss mice (n=14 each) were detected under stress conditions using forced swimming test and tail suspension test.Open field test was used to detect the spontaneous motility of two kinds of mice.Results The immobility time of C57BL/6J mice was significantly longer than that of NIH Swiss mice [ (133.198 ± 5.749) s vs (80.265± 10.939) s,P=0.000] in forced swimming test,which was contrast to that in tail suspension test [ (151.315±12.161)s vs (95.107±14.649)s,P=0.007].Howevcr in open field test,no diffcrcncc was found in total distance,standing number,movement time,total distance in fringe area,movement time in fringe area,total distance in central area and movement time in central area between the two kinds of mice.Conclusion C57BL/6J mice are easier to show the depressive-like behavior under stress conditions,while NIH Swiss mice show a certain of depression resistance.These different behavioral manifestations in two different kinds of mice may be caused by genetic factors.
