中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2010年
7期
474-477
,共4页
胡婕%魏仲香%姜奕%梁再赋%耿龙
鬍婕%魏仲香%薑奕%樑再賦%耿龍
호첩%위중향%강혁%량재부%경룡
天疱疮%桥粒芯糖蛋白质3%T淋巴细胞%Th1细胞%Th2细胞
天皰瘡%橋粒芯糖蛋白質3%T淋巴細胞%Th1細胞%Th2細胞
천포창%교립심당단백질3%T림파세포%Th1세포%Th2세포
Pemphigus%Desmoglein 3%T-lymphocytes%Th1 cells%Th2 cells
目的 观察桥粒芯糖蛋白3(Dsg3)对寻常型天疱疮(PV)患者外周血T淋巴细胞增殖的影响.方法 PV患者12例,正常人22例,提取外周血单一核细胞(PBMC),采用流式细胞仪技术分别检测Dsg3刺激下PBMC中T淋巴细胞亚群变化及T淋巴细胞增殖情况,分析PV患者与正常人之间存在的差异.结果 PV患者PBMC在Dsg3刺激下Th2型T淋巴细胞所占比例为12.17%±5.32%,Th1型T淋巴细胞所占比例为4.08%±1.50%;未经Dsg3刺激的阴性对照组分别为9.84%±5.41%和3.91%±1.38%.PV患者PBMC在Dsg3刺激和无Dsg3刺激时的Th2型T淋巴细胞所占比例均显著高于正常人(P<O.05);Dsg3刺激下PV患者T淋巴细胞发生增殖,CD4+T淋巴细胞增殖率为4.65%±3.28%,显著高于正常人(P<0.05). 结论 Dsg3可诱导PV患者CD4+T淋巴细胞发生特异性增殖,且主要为Th2优势型.
目的 觀察橋粒芯糖蛋白3(Dsg3)對尋常型天皰瘡(PV)患者外週血T淋巴細胞增殖的影響.方法 PV患者12例,正常人22例,提取外週血單一覈細胞(PBMC),採用流式細胞儀技術分彆檢測Dsg3刺激下PBMC中T淋巴細胞亞群變化及T淋巴細胞增殖情況,分析PV患者與正常人之間存在的差異.結果 PV患者PBMC在Dsg3刺激下Th2型T淋巴細胞所佔比例為12.17%±5.32%,Th1型T淋巴細胞所佔比例為4.08%±1.50%;未經Dsg3刺激的陰性對照組分彆為9.84%±5.41%和3.91%±1.38%.PV患者PBMC在Dsg3刺激和無Dsg3刺激時的Th2型T淋巴細胞所佔比例均顯著高于正常人(P<O.05);Dsg3刺激下PV患者T淋巴細胞髮生增殖,CD4+T淋巴細胞增殖率為4.65%±3.28%,顯著高于正常人(P<0.05). 結論 Dsg3可誘導PV患者CD4+T淋巴細胞髮生特異性增殖,且主要為Th2優勢型.
목적 관찰교립심당단백3(Dsg3)대심상형천포창(PV)환자외주혈T림파세포증식적영향.방법 PV환자12례,정상인22례,제취외주혈단일핵세포(PBMC),채용류식세포의기술분별검측Dsg3자격하PBMC중T림파세포아군변화급T림파세포증식정황,분석PV환자여정상인지간존재적차이.결과 PV환자PBMC재Dsg3자격하Th2형T림파세포소점비례위12.17%±5.32%,Th1형T림파세포소점비례위4.08%±1.50%;미경Dsg3자격적음성대조조분별위9.84%±5.41%화3.91%±1.38%.PV환자PBMC재Dsg3자격화무Dsg3자격시적Th2형T림파세포소점비례균현저고우정상인(P<O.05);Dsg3자격하PV환자T림파세포발생증식,CD4+T림파세포증식솔위4.65%±3.28%,현저고우정상인(P<0.05). 결론 Dsg3가유도PV환자CD4+T림파세포발생특이성증식,차주요위Th2우세형.
Objective To investigate the impact of desmoglein 3 (Dsg3) on the proliferation of peripheral T lymphocytes from patients with pemphigus vulgaris (PV).Methods Peripheral blood mononuclear cells (PBMCs) were obtained from 12 patients with PV and 22 normal human controls,cultured with or without the presence of Dsg3 or phytohemagglutinin for 3 days.Flow cytometry was performed to detect the changes of T-lymphocyte subsets in PBMCs stimulated with Dsg3 and proliferation of T-lymphocytes.Results In patients with PV,the percentage of Th2 and Th1 cells was 12.17%±5.32% and 4.08%±1.50%,respectively in Dsg3stimulated PBMCs,9.84%±5.41% and 3.91%±1.38%,respectively in non-stimulated PBMCs.Increased percentage of Th2 cells was observed in Dsg3-stimulated and non-stimulated PBMCs from patients with PV compared with those from normal human controls (both P<0.05).After stimulation with Dsg3,there was a significant proliferation of T cells from patients,and the proliferation rate of CD4+T cells was 4.65%±3.28%,which was significantly higher than that from normal controls(P<0.05).Conclusion Dsg3 can induce the specific proliferation of CD4+T cells,especially Th2 type CD+4 T cells,from patients with PV.
