CAJ | 학술논문

将雄性4周龄SD大鼠分为正常饲料组和高脂饲料组,喂养10周后分别以二甲双胍治疗(200mg/kg)6周.以正常血糖-高胰岛素钳夹试验评估胰岛素敏感性,留取肾周脂肪组织,采用实时定量PCR及Western印迹法检测脂肪组织中chemerin mRNA和蛋白的表达水平.结果显示,高脂饲料诱导的胰岛素抵抗、大鼠肾周脂肪组织chemerin mRNA和蛋白水平高于正常饲料喂养大鼠(均P＜0.05),二甲双胍治疗6周后明显降低(均P＜0.01).脂肪组织中chemerin mRNA、蛋白表达水平均与附睾脂肪质量指数显著相关(均P＜0.05).
장웅성4주령SD대서분위정상사료조화고지사료조,위양10주후분별이이갑쌍고치료(200mg/kg)6주.이정상혈당-고이도소겸협시험평고이도소민감성,류취신주지방조직,채용실시정량PCR급Western인적법검측지방조직중chemerin mRNA화단백적표체수평.결과현시,고지사료유도적이도소저항、대서신주지방조직chemerin mRNA화단백수평고우정상사료위양대서(균P＜0.05),이갑쌍고치료6주후명현강저(균P＜0.01).지방조직중chemerin mRNA、단백표체수평균여부고지방질량지수현저상관(균P＜0.05).
After 4 weeks old male Sprague-Dawley rats were fed with normal diet and high-fat diet for 10 weeks,the rats were treated with metformin (200 mg/kg) for 6 weeks. Insulin sensitivity was determined by hyperinsulinemic-euglycemic clamp technique.Realtime PCR and western blot were used to measure mRNA and protein levels of chemerin in perirenal adipose tissue of rats.The results showed that the expressions of chemerin mRNA and protein were higher in high-fat diet-induced insulin resistant rats compared with rats fed with normal diet ( both P＜0.05 ),and these incremental findings were diminished by metformin treatment ( both P＜0.01 ).The levels of chemerin mRNA and protein were correlated well with the epididymis fat mass index.