CAJ | 학술논문

在F344雄性大鼠腹腔注射血蓝蛋白(KLH),研究T-细胞依赖抗原初始免疫反应对中枢神经系统5-羟色胺(5-HT)代谢的影响.当免疫刺激大鼠4天后,其下丘脑匀浆5-羟色胺水平出现降低,同时应用在体脑微透析技术研究发现清醒大鼠腹腔注射血蓝蛋白4天后,其下丘脑前叶细胞外液的5-羟色胺水平增加.为了评价在免疫反应中大鼠下丘脑5-羟色胺释放效应,本文应用氯苯丙胺(PCA)选择性预先耗竭5-羟色胺释放,并测定这些动物在注射血蓝蛋白后抗体产生情况.结果显示在应用氯苯丙胺预处理的血蓝蛋白免疫动物特异抗体IgM和IgG与未用氯苯丙胺预处理的动物比较均有明显增加(P＜0.01).上述资料表明,对于T-细胞依赖抗原,其初始抗体产生的量是由位于下丘脑5-羟色胺神经终端抑制性递质释放所调节.
재F344웅성대서복강주사혈람단백(KLH),연구T-세포의뢰항원초시면역반응대중추신경계통5-간색알(5-HT)대사적영향.당면역자격대서4천후,기하구뇌균장5-간색알수평출현강저,동시응용재체뇌미투석기술연구발현청성대서복강주사혈람단백4천후,기하구뇌전협세포외액적5-간색알수평증가.위료평개재면역반응중대서하구뇌5-간색알석방효응,본문응용록분병알(PCA)선택성예선모갈5-간색알석방,병측정저사동물재주사혈람단백후항체산생정황.결과현시재응용록분병알예처리적혈람단백면역동물특이항체IgM화IgG여미용록분병알예처리적동물비교균유명현증가(P＜0.01).상술자료표명,대우T-세포의뢰항원,기초시항체산생적량시유위우하구뇌5-간색알신경종단억제성체질석방소조절.
To study the effects of a primary immune response to T-cell dependent antigens on central serotonin(5-HT)metabolism,keyhole limpet hemocyanin(KLH)was administered intraperitoneally to F344 male rats. A decrease in hypothalamic 5-HT levels was observed on Day 4 following stimulation of the immune system. In the anterior hypothalamus, a concomitant increase in the basal extracellular 5-HT levels was measured by using in vivo microdialysis in awake rats on Day 4 following KLH administration.Furthermore,to evaluate the consequence of this 5-HT release on the immune response, we selectively denervated serotonin projections with p-chloroan-phetamine (PCA)and measured antibody production in these animals after KLH injection. The production of specific anti-KLH antibodies of IgM and IgG subclasses in the plasma was significantly increased in PCA-treated animals immunized with KLH compared to KLH-injected rats on Days 5,6 and 7 for IgM and Day 6 and 7 for IgG. Our data indicate that the magnitude of the primary antibody resopnses to T-cell antigens such as KLH are subject to tonic inhibitory modulation by serotoninergic nerve terminals located in the anterior hypothalamus.