分析试验室
分析試驗室
분석시험실
ANALYTICAL LABORATORY
2009年
8期
55-58
,共4页
高速毛细管电泳%安培检测%甲巯咪唑
高速毛細管電泳%安培檢測%甲巰咪唑
고속모세관전영%안배검측%갑구미서
High-speed capillary electrophoresis%Amperometric detection%Thiamazole
采用高速毛细管电泳安培法对甲巯咪唑(TMZ)及其制剂的测定进行了研究; 通过优化检测电位、毛细管长度和内径、分离电压、缓冲溶液等实验参数, TMZ在60 s内可以得到较好的分离, 线性范围在2.00×10-3～2.80×10-5 mol/L, 检出限为3.50×10-6 mol/L; 峰面积和迁移时间的相对标准偏差分别为2.7%、 1.2% (n＝8); 该法可用于制剂中TMZ的检测.
採用高速毛細管電泳安培法對甲巰咪唑(TMZ)及其製劑的測定進行瞭研究; 通過優化檢測電位、毛細管長度和內徑、分離電壓、緩遲溶液等實驗參數, TMZ在60 s內可以得到較好的分離, 線性範圍在2.00×10-3～2.80×10-5 mol/L, 檢齣限為3.50×10-6 mol/L; 峰麵積和遷移時間的相對標準偏差分彆為2.7%、 1.2% (n＝8); 該法可用于製劑中TMZ的檢測.
채용고속모세관전영안배법대갑구미서(TMZ)급기제제적측정진행료연구; 통과우화검측전위、모세관장도화내경、분리전압、완충용액등실험삼수, TMZ재60 s내가이득도교호적분리, 선성범위재2.00×10-3～2.80×10-5 mol/L, 검출한위3.50×10-6 mol/L; 봉면적화천이시간적상대표준편차분별위2.7%、 1.2% (n＝8); 해법가용우제제중TMZ적검측.
The characteristic of high-speed capillary electrophoresis (HSCE) lies in the feasibility to improve the separation speed within several seconds or minutes by increasing the voltage and using short length and narrow inner diameter capillary. A method based on high-speed capillary electrophoresis with amperometric detection was developed for the determination of thiamazole (TMZ) and its tablets. The effects of some important factors, such as the detection potential, the length and the inner diameter of capillary, the separation voltage and the running buffer on separation were investigated to acquire the optimum conditions. Under the optimum conditions, TMZ was satisfactorily eluted in 60 second with the detection limit as low as 3.50×10-6 mol/L. The response was linear in the range of 2.00×10-3～2.80×10-5 mol/L. The relative standard deviations (n=8) of peak area and migration time were 2.7% and 1.2%, respectively. The method was applied to the determination of TMZ in the tablets with satisfactory results.
