时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
11期
2675-2677
,共3页
梅志刚%朱兵%何伟%高昕妍
梅誌剛%硃兵%何偉%高昕妍
매지강%주병%하위%고흔연
耳针%孤束核%迷走神经运动背核%辣根过氧化物酶
耳針%孤束覈%迷走神經運動揹覈%辣根過氧化物酶
이침%고속핵%미주신경운동배핵%랄근과양화물매
Auricular acupuncture%Nucleus of solitary tract%Dorsal nucleus of vagus nerve%Horseradish peroxidase(HRP)
目的 观察支配耳甲区迷走神经在延髓水平中枢的投射情况,探讨耳针作用机理.方法 健康成年SD大鼠10只,外耳道口方向分离耳甲皮肤与皮下组织,微量注射器缝内注射40 μl 浓度为30%的HRP神经示踪剂,神经示踪技术观察标记细胞在延脑水平核团分布情况.结果 除了在三叉神经脊束核外,在孤束核和迷走神经运动背核以及其他核团也发现了标记神经元或神经纤维.结论 耳甲区迷走神经分支与迷走初级中枢有神经联系,耳甲穴位针刺效应很可能是通过耳-迷走反射途径实现的.
目的 觀察支配耳甲區迷走神經在延髓水平中樞的投射情況,探討耳針作用機理.方法 健康成年SD大鼠10隻,外耳道口方嚮分離耳甲皮膚與皮下組織,微量註射器縫內註射40 μl 濃度為30%的HRP神經示蹤劑,神經示蹤技術觀察標記細胞在延腦水平覈糰分佈情況.結果 除瞭在三扠神經脊束覈外,在孤束覈和迷走神經運動揹覈以及其他覈糰也髮現瞭標記神經元或神經纖維.結論 耳甲區迷走神經分支與迷走初級中樞有神經聯繫,耳甲穴位針刺效應很可能是通過耳-迷走反射途徑實現的.
목적 관찰지배이갑구미주신경재연수수평중추적투사정황,탐토이침작용궤리.방법 건강성년SD대서10지,외이도구방향분리이갑피부여피하조직,미량주사기봉내주사40 μl 농도위30%적HRP신경시종제,신경시종기술관찰표기세포재연뇌수평핵단분포정황.결과 제료재삼차신경척속핵외,재고속핵화미주신경운동배핵이급기타핵단야발현료표기신경원혹신경섬유.결론 이갑구미주신경분지여미주초급중추유신경련계,이갑혈위침자효응흔가능시통과이-미주반사도경실현적.
Objective To observe projections of nervus auricularis vagi in brain stem of oblongata level,and to study the mechanism of auricular acupuncture. Methods Ten SD rats were used in this experiment, a skin incision was performed in the region of auricular concha of each rat, forty microliters of a 30% solution of horseradish peroxidase (HRP) in normal saline were subcuta-neously injected into the cut central end of auricular branch of vagus nerve, then distribution of the labeled neurons in the primary brain stem was observed using HRP retrograde tracing technique. Results Labeled neurons and fibers were seen in the nucleus of solitary tract, dorsal nucleus of vagus nerve and other nucleus besides nucleus of spinal tract of trigeminal. Conclusion There exists neural projection to NTS and DMV from the terminal of auricular vagus branch, maybe effect of auricular acupuncture is completed through auricular - vagal reflexes.
