CAJ | 학술논문

目的检测非小细胞肺癌(NSCLC)患者肿瘤间质T淋巴细胞亚群分布.探讨NSCLC临床分期与淋巴细胞各亚群比例及免疫功能的关系.方法收集60例NSCLC患者新鲜肿瘤标本,运用免疫组化染色方法检测T淋巴细胞哑群CD4~+、CD8~+和CD4~+ CD25~+ Foxp3~+ (Treg)的分布.结果与Ⅰ/Ⅱ期患者比较,Ⅲ/Ⅳ期患者肿瘤组织中CD4~+和CD4~+/CD8~+比值下降(P<0.05),CD8~+和CD4~+ CD25~+ Foxp3~+比值升高(P<0.05).较之癌旁组织,Treg细胞在肿瘤组织中明显富集.结论 NSCLC患者T淋巴细胞免疫功能受抑制.作为主要免疫抑制细胞的Treg细胞比例与肿瘤分期呈正相关.
목적 검측비소세포폐암(NSCLC)환자종류간질T림파세포아군분포.탐토NSCLC림상분기여림파세포각아군비례급면역공능적관계.방법 수집60례NSCLC환자신선종류표본,운용면역조화염색방법검측T림파세포아군CD4~+、CD8~+화CD4~+ CD25~+ Foxp3~+ (Treg)적분포.결과 여Ⅰ/Ⅱ기환자비교,Ⅲ/Ⅳ기환자종류조직중CD4~+화CD4~+/CD8~+비치하강(P<0.05),CD8~+화CD4~+ CD25~+ Foxp3~+비치승고(P<0.05).교지암방조직,Treg세포재종류조직중명현부집.결론 NSCLC환자T림파세포면역공능수억제.작위주요면역억제세포적Treg세포비례여종류분기정정상관.
Objective To study the relation of tumor interstitial T lymphocyte subset activity to the clinical staging of non-small-cell lung cancer (NSCLC) and the immune response.Methods Immunohistochemical staining for CD~+, CD8~+ and CD4~+CD25~+ Foxp3~+ (regulatory T cells, Treg) T cells was performed on paraffin-embedded tissues from 60 NSCLC cases.Results Compared to stage Ⅰ/Ⅱ NSCLC patients, patients in stage Ⅲ/Ⅳ showed a significant decrease in the percentage of CD4~+ and CD4~+/CD8~+ T cells (P<0.05) and an increase in CD8~+ and CD4~+ CD25~+ Foxp3~+ T cells (P<0.05).Treg cells were enriched in the tumor tissue as compared with those in the adjacent tissues.Conclusion The proportion of CD4~+ CD25~+ Foxp3~+ Treg cells is positively correlated to the clinical staging of NSCLC, in which T cell-mediated immune response is suppressed.