CAJ | 학술논문

研究α2,8-唾液酸转移酶VI(Sixth type of α2,8-sialyltransferase, ST8Sia VI) 对乳腺癌细胞生物学功能的影响及其作用的分子机制.采用全基因组芯片技术检测ST8Sia VI过表达前后小鼠乳腺癌细胞4T1基因表达谱的差异;利用PathwayExplorer分析ST8Sia VI过表达前后对基因网络的影响.实验结果表明:ST8Sia VI过表达引起201个基因表达有差异,其中22个基因与肿瘤细胞的黏附、生长、运动、免疫和周期有关.另外,PathwayExplorer分析结果显示,差异表达基因涉及的最显著变化的基因网络是"依赖β-arrestin 的G蛋白偶联受体(GPCR)信号通路";进一步的实验结果证明该通路下游Raf蛋白的磷酸化水平在ST8Sia VI过表达细胞显著升高.上述结果为ST8Sia VI生物学功能的深入研究提供了前提.
연구α2,8-타액산전이매VI(Sixth type of α2,8-sialyltransferase, ST8Sia VI) 대유선암세포생물학공능적영향급기작용적분자궤제.채용전기인조심편기술검측ST8Sia VI과표체전후소서유선암세포4T1기인표체보적차이;이용PathwayExplorer분석ST8Sia VI과표체전후대기인망락적영향.실험결과표명:ST8Sia VI과표체인기201개기인표체유차이,기중22개기인여종류세포적점부、생장、운동、면역화주기유관.령외,PathwayExplorer분석결과현시,차이표체기인섭급적최현저변화적기인망락시"의뢰β-arrestin 적G단백우련수체(GPCR)신호통로";진일보적실험결과증명해통로하유Raf단백적린산화수평재ST8Sia VI과표체세포현저승고.상술결과위ST8Sia VI생물학공능적심입연구제공료전제.
This study aimed at clarifying the roles and molecular mechanisms of the sixth type of sialyltransferase(ST8Sia VI) in breast cancer cells. In this study, the gene expression of 4T1 cells over-expressed ST8Sia VI and 4T1 cells transfected empty vector were compared by gene chip, and the related signal pathways of differentially expressed genes were determined based on the public databases with PathwayExplorer. The results showed that the expressions of 201 genes were differential in ST8Sia VI over-expressed cells and control cells, of which 22 genes were involved in cell adhesion, growth, locomotion, immune process and cell cycle. The genes expressed differentially were also involved in β-arrestin-dependent GPCR signaling pathway. In addition, Raf, a downstream member of β-arrestin-dependent GPCR signaling pathway, was found to be highly phosphorylated in 4T1-ST8Sia VI cells. These results lay the foundation for an in-depth study of ST8Sia VI.