CAJ | 학술논문

采用实时荧光定量RT-PCR和Northern blotting技术检测了野生型拟南芥中CBP60g基因对丁香假单胞菌和非生物胁迫的响应,并对丁香假单胞菌接种后,野生型拟南芥、cbp60g-1突变体和CBP60g过表达转基因植物中抗逆相关基因的表达变化进行检测.结果显示:(1)在野生型拟南芥中CBP60g基因的表达能被丁香假单胞菌、高盐、冷和机械损伤所诱导.(2)经丁香假单胞菌诱导后病程相关基因PR5和AIG1的表达在过表达转基因植物中明显高于野生型.(3)受干旱和ABA诱导的AtMYB2基因的表达在过表达转基因植物中也高于野生型.研究表明,CBP60g同时参与了拟南芥对生物和非生物胁迫响应.
채용실시형광정량RT-PCR화Northern blotting기술검측료야생형의남개중CBP60g기인대정향가단포균화비생물협박적향응,병대정향가단포균접충후,야생형의남개、cbp60g-1돌변체화CBP60g과표체전기인식물중항역상관기인적표체변화진행검측.결과현시:(1)재야생형의남개중CBP60g기인적표체능피정향가단포균、고염、랭화궤계손상소유도.(2)경정향가단포균유도후병정상관기인PR5화AIG1적표체재과표체전기인식물중명현고우야생형.(3)수간한화ABA유도적AtMYB2기인적표체재과표체전기인식물중야고우야생형.연구표명,CBP60g동시삼여료의남개대생물화비생물협박향응.
In this study,real-time PCR or Northern blotting was employed to analyze the expression patterns of the CBP60g gene in wild-type Arabidopsis thaliana after Pseudomonas syringae and abiotic stress treatment.In addition,the expression of pathogen-induced genes in wild-type,cbp60g-1 mutant and CBP60g overexpression plants after P.syringae inoculation were compared.The results showed:(1)Transcript of CBP60g was induced by P.syringae,high salt,cold and wounding treatment in wild-type plants.(2)The expression of pathogen-induced genes PR5 and AIG1 in the CBP60g overexpression plants was enhanced after P.syringae inoculation and was reduced in the mutant.(3)AtMYB2,an abiotic-stress induced gene reported previously,was also induced by Pst DC3000,and reached to a higher level in overexpression plants than in wild-type.Taken together,these results indicate that CBP60g involved in A.thaliana multiple stresses response.