CAJ | 학술논문

目的观察川芎嗪对类风湿性关节炎(RA)模型大鼠RANK/RANKL/OPG 在外周血中CD3~+T淋巴细胞、中性粒细胞、CD14单核细胞的表达率及平均荧光强度,探讨川芎嗪在RA骨破坏和炎症过程中的意义.方法大鼠随机分成5组,正常对照组、模型对照组、川芎嗪大剂量组、川芎嗪小剂量组、阳性药物对照组,每组10只.给药7 d后,应用间接免疫荧光标记和流式细胞技术对各组大鼠外周血相关指标进行检测分析.结果与正常对照组相比,RA大鼠骨保护素(OPG)表达明显降低,从正常的24.7降至18.7(q=4.2,P<0.05),RANK、RANKL.变化不明显,经过川芎嗪治疗后,大剂量组OPG有明显的回升,至23.8%(q=3.97,P<0.05),小剂量组变化不明显.RANK在CD3~+细胞、中性粒细胞、CD14单核细胞上的平均荧光强度明显降低,分别为20.6、135.4、84.2,经川芎嗪大剂量治疗后明显升高,分别达到31.0、192.1、95.6(q=10.4、q=8.6、q=6.3,P<0.05).结论大剂量川芎嗪可以通过调节OPG/RANK/RANKL.途径对RA起一定的作用.
목적 관찰천궁진대류풍습성관절염(RA)모형대서RANK/RANKL/OPG 재외주혈중CD3~+T림파세포、중성립세포、CD14단핵세포적표체솔급평균형광강도,탐토천궁진재RA골파배화염증과정중적의의.방법 대서수궤분성5조,정상대조조、모형대조조、천궁진대제량조、천궁진소제량조、양성약물대조조,매조10지.급약7 d후,응용간접면역형광표기화류식세포기술대각조대서외주혈상관지표진행검측분석.결과여정상대조조상비,RA대서골보호소(OPG)표체명현강저,종정상적24.7강지18.7(q=4.2,P<0.05),RANK、RANKL.변화불명현,경과천궁진치료후,대제량조OPG유명현적회승,지23.8%(q=3.97,P<0.05),소제량조변화불명현.RANK재CD3~+세포、중성립세포、CD14단핵세포상적평균형광강도명현강저,분별위20.6、135.4、84.2,경천궁진대제량치료후명현승고,분별체도31.0、192.1、95.6(q=10.4、q=8.6、q=6.3,P<0.05).결론 대제량천궁진가이통과조절OPG/RANK/RANKL.도경대RA기일정적작용.
Objective To observe the expression rate and the average fluorescence intensity of receptor activator of NF-kB (RANK), RANK ligand (RANKL) , Osteoprotegerin (OPG) of CD3~+ T lymphocytes, neu-trophils, and CD14 monocyte in the peripheral blood of rheumatoid arthritis (RA) model rats and to explore the significance of bone destruction and inflammation in the process of RA by using Ligustrazine. Methods Rats were randomly divided into five groups, the normal group, the model control group, high dosage Ligustrazine group, low dosage Ligustrazine group and positive drug group. Each group had ten animals. After 7 days, peripheral blood of rats was examined by indirect immunofluorescence and flow cytometry technology. Results The OPG expression was decreased significantly from 24.7% to 18.7% compared with the control group (q=4.2, P<0.05) and increased to 23. 8%(g=3.97, P<0.05) after high-dose Ligustrazine treatment, but the expression of RANK and RANKL did not change significantly in RA rats treated with high-dose and low-dose ligustrazine group. The average fluorescence intensity of RANK in CD3~+ T cells, neutrophils, and CD14 monocytes in RA rats decreased significantly to 20.6, 135.4 and 84.2, respectively, but increased after high-dose ligustrazine treatment to 31.0, 192.1 and 95.6 (q = 10. 4, q =8. 6, q=6.3, P<0.05). Conclusion High-dose ligustrazine plays a role on RA by regulating the RANK/RANKL/ OPG pathway.