CAJ | 학술논문

目的探讨低剂量率中子长期照射对大鼠外周血细胞亚群的影响.方法 96只雄性大鼠分为对照组和照射组,照射组每天用低剂量率中子252 Cf(吸收剂量率为0.35 mGy/h)照射20.5 h,在照射的第14、28、42、56和70天(累积剂量分别为0.1、0.2、0.3、0.4和0.5 Gy)及停止照射后第35天各取8只大鼠,用血细胞计数仪检测大鼠外周血WBC、用流式细胞仪检测外周血CD4+ CD3+、CD8+CD3+、CD45RA+/CD161α+亚群的变化.结果累积剂量为0.3、0.4及0.5 Gy时WBC明显低于对照组(P<0.05),停止照射后35 d,WBC显著低于对照组(P<0.01);累积剂量为0.1、0.3、0.4、0.5 Gy及停止照射后35 d,外周血CD4+ CD3-细胞比例显著高于对照组(P<0.01或<0.05);累积剂量为0.2和0.3 Gy时CD8+CD3-细胞比例显著高于对照组(P<0.05或P<0.01).而累积剂量为0.1 Gy时的CD44 CD3-细胞比例及0.1和0.2 Gy时的CD8+ CD3+细胞比例明显高于同一天对照组(P<0.01或<0.05).另外,低剂量率中子长期照射可使累积剂量为0.2～0.3 Gy的外周血NK细胞(CD161α+CD45RA-)显著升高,累积剂量为0.1～0.5 Gy及停止照射后35 d照射组的外周血B细胞(CD161α-CD45RA+)比例明显下降.结论低剂量率裂变中子长期照射可使外周血淋巴细胞TCR基因突变,使大鼠外周血WBC减少,淋巴细胞中B细胞减少,NK细胞细胞比例升高,这种变化在停止照射后一段时间仍可能难以恢复.
목적 탐토저제량솔중자장기조사대대서외주혈세포아군적영향.방법 96지웅성대서분위대조조화조사조,조사조매천용저제량솔중자252 Cf(흡수제량솔위0.35 mGy/h)조사20.5 h,재조사적제14、28、42、56화70천(루적제량분별위0.1、0.2、0.3、0.4화0.5 Gy)급정지조사후제35천각취8지대서,용혈세포계수의검측대서외주혈WBC、용류식세포의검측외주혈CD4+ CD3+、CD8+CD3+、CD45RA+/CD161α+아군적변화.결과 루적제량위0.3、0.4급0.5 Gy시WBC명현저우대조조(P<0.05),정지조사후35 d,WBC현저저우대조조(P<0.01);루적제량위0.1、0.3、0.4、0.5 Gy급정지조사후35 d,외주혈CD4+ CD3-세포비례현저고우대조조(P<0.01혹<0.05);루적제량위0.2화0.3 Gy시CD8+CD3-세포비례현저고우대조조(P<0.05혹P<0.01).이루적제량위0.1 Gy시적CD44 CD3-세포비례급0.1화0.2 Gy시적CD8+ CD3+세포비례명현고우동일천대조조(P<0.01혹<0.05).령외,저제량솔중자장기조사가사루적제량위0.2～0.3 Gy적외주혈NK세포(CD161α+CD45RA-)현저승고,루적제량위0.1～0.5 Gy급정지조사후35 d조사조적외주혈B세포(CD161α-CD45RA+)비례명현하강.결론 저제량솔렬변중자장기조사가사외주혈림파세포TCR기인돌변,사대서외주혈WBC감소,림파세포중B세포감소,NK세포세포비례승고,저충변화재정지조사후일단시간잉가능난이회복.
Objective To evaluate the effects of long-term, low dose rate fission neutron irradiation on lymphocyte subpopulations in peripheral blood of rots. Methods Ninety-six rats were randomly divided into control group and irradiated group exposed to low dose rate fission neutron (252 Cf,0.35 mGy/h) for 20.5 h every day.At days 14,28,42,56 and 70 d after irradiation and 35 d after stopping irradiation, After 8 rats of each group were killed, WBC and lymphocyte subpopulations of CD4+ CD3+,CD8+ CD3+ and CD45RA+/CD161α+ in peripheral blood were estimated respectively. Results Compared with the control group,WBC was reduced significantly at dose of 0.3, 0.4 and 0.5 Gy (P<0.05), and was decreased remarkably at 35 d after stopping irradiation (P<0.01). The rate of CD4+ CD3- was evidently higher compared with control group at doses of 0.1,0.3, 0.4 and 0.5 Gy and 35 d after stopping irradiation (P<0.01, P<0.01 and P < 0.05), and the rate of CD8+ CD3- was obviously higher compared with control group at dose of 0.2 and 0.3 Gy (P<0.05 and P<0.01). On the other hand, the rate of CD4+ CD3+ at dose of 0.1 Gy ( P < 0.01 ) and the rate of CD8+ CD3+ at doses of 0.1 and 0.2 Gy (P <0.05) were higher when compared with control group. At the same time,peripheral blood NK cells(CD161α+ CD45RA-) was increased significantly at doses of 0.2-0.3 Gy, and peripheral blood B cells(CD161α- CD45RA+ ) was reduced remarkably at doses of 0.1-0.5 Gy and 35 d after stopping irradiation compared with the control group. Conclusions Long-term irradiation with low dose rate fission neutron could make TCR (T-cell-receptor) mutant, therefore, WBC, B cells in peripheral blood significantly reduced and NK cells increased. These changes may could not recover at 35 d after stopping irradiation.