中华超声影像学杂志
中華超聲影像學雜誌
중화초성영상학잡지
CHINESE JOURNAL OF ULTRASONOGRAPHY
2010年
3期
251-254
,共4页
徐上妍%詹维伟%朱樱%周建桥
徐上妍%詹維偉%硃櫻%週建橋
서상연%첨유위%주앵%주건교
超声检查%微气泡%内皮细胞%一氧化氮%一氧化氮合酶
超聲檢查%微氣泡%內皮細胞%一氧化氮%一氧化氮閤酶
초성검사%미기포%내피세포%일양화담%일양화담합매
Ultrasound%Microbubble%Endothelial cells%Nitric oxide%Nitric oxide synthase
目的 评估诊断性超声联合微泡对内皮细胞中内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)及一氧化氮(nitric oxide,NO)生成的增强效应.方法 将正常培养的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)分为空白对照组(A组)、纯微泡组(B组)、单纯超声组(C组)和超声联合微泡组(D组).其中,D组按条件不同又分为:辐照时间不同组(1 min、5 min、10 min)、机械指数不同组(0.09、0.4、1.0)、微泡浓度不同组(5×10~8/ml、2.5×10~8/ml、1.25×10~8/ml).干预后即刻和干预后24 h分别在光镜下观察细胞形态结构,RT-PCR测细胞eNOS相对表达量,NO试剂盒测培养液中NO水平,并用统计学方法对上述指标进行组间比较.结果 D组中eNOS及NO含量明显较其他三组高;当参数选择为机械指数1.0、微泡浓度2.5×10~8/ml、辐照时间10 min时,D组中eNOS和NO的增加程度最明显;并且干预后即刻和干预后24 h各组细胞及细胞膜形态结构在光镜下均无显著变化.结论 超声联合微泡辐照能增加内皮细胞中eNOS和NO生成;给予的超声及微泡条件不同,其增强效果也不同.
目的 評估診斷性超聲聯閤微泡對內皮細胞中內皮型一氧化氮閤酶(endothelial nitric oxide synthase,eNOS)及一氧化氮(nitric oxide,NO)生成的增彊效應.方法 將正常培養的人臍靜脈內皮細胞(human umbilical vein endothelial cells,HUVEC)分為空白對照組(A組)、純微泡組(B組)、單純超聲組(C組)和超聲聯閤微泡組(D組).其中,D組按條件不同又分為:輻照時間不同組(1 min、5 min、10 min)、機械指數不同組(0.09、0.4、1.0)、微泡濃度不同組(5×10~8/ml、2.5×10~8/ml、1.25×10~8/ml).榦預後即刻和榦預後24 h分彆在光鏡下觀察細胞形態結構,RT-PCR測細胞eNOS相對錶達量,NO試劑盒測培養液中NO水平,併用統計學方法對上述指標進行組間比較.結果 D組中eNOS及NO含量明顯較其他三組高;噹參數選擇為機械指數1.0、微泡濃度2.5×10~8/ml、輻照時間10 min時,D組中eNOS和NO的增加程度最明顯;併且榦預後即刻和榦預後24 h各組細胞及細胞膜形態結構在光鏡下均無顯著變化.結論 超聲聯閤微泡輻照能增加內皮細胞中eNOS和NO生成;給予的超聲及微泡條件不同,其增彊效果也不同.
목적 평고진단성초성연합미포대내피세포중내피형일양화담합매(endothelial nitric oxide synthase,eNOS)급일양화담(nitric oxide,NO)생성적증강효응.방법 장정상배양적인제정맥내피세포(human umbilical vein endothelial cells,HUVEC)분위공백대조조(A조)、순미포조(B조)、단순초성조(C조)화초성연합미포조(D조).기중,D조안조건불동우분위:복조시간불동조(1 min、5 min、10 min)、궤계지수불동조(0.09、0.4、1.0)、미포농도불동조(5×10~8/ml、2.5×10~8/ml、1.25×10~8/ml).간예후즉각화간예후24 h분별재광경하관찰세포형태결구,RT-PCR측세포eNOS상대표체량,NO시제합측배양액중NO수평,병용통계학방법대상술지표진행조간비교.결과 D조중eNOS급NO함량명현교기타삼조고;당삼수선택위궤계지수1.0、미포농도2.5×10~8/ml、복조시간10 min시,D조중eNOS화NO적증가정도최명현;병차간예후즉각화간예후24 h각조세포급세포막형태결구재광경하균무현저변화.결론 초성연합미포복조능증가내피세포중eNOS화NO생성;급여적초성급미포조건불동,기증강효과야불동.
Objective To assess the enhancement effect of diagnostic ultrasound combined with microbubbles in the generation of endothelial nitric oxide synthase(eNOS)and nitric Oxide(NO)in endothelial cells.Methods Normal cultured human umbilical vein endothelial cells(HUVEC)were divided into blank control group(A group),simple microbubble group(B group),simple ultrasound group(C group)and ultrasound combined with microbubble group(D group).According to different conditions,group D was divided into three sub-groups:different time groups(1 min,5 min,10 min);different machinery index(MI)groups(0.09,0.4,1.0),different microbubble concentration groups(5×10~8/ml,2.5×10~8/ml,1.25×10~8/ml).Cell morpha was observed in the light microscope immediately and 24 h after the intervention,respectively.RT-PCR was used to measure the relative expression of eNOS in cells.NO kit was used to measure the NO Ievels in culture medium.And statistical methods were used to analyse the experimental data.Results NO and eNOS were significantly higher in group D than the other three groups.When MI=1.0,microbubble concentration=2.5×10~8/ml,and irradiation time=10 min,the increase of eNOS and NO in group D was the most obvious.Furthermore,the cell morphology had no significant change in the light microscope immediately and 24 h after the intervention.Conclusions Ultrasound combined with microbubble can increase the generation of eNOS and NO in endothelial cells.
