中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2010年
12期
973-976
,共4页
孙任成%田克立%马忠兵%傅勤烨%张强%高德宗%李玉阳%李亮%余之刚
孫任成%田剋立%馬忠兵%傅勤燁%張彊%高德宗%李玉暘%李亮%餘之剛
손임성%전극립%마충병%부근엽%장강%고덕종%리옥양%리량%여지강
乳腺肿瘤%胸苷酸合酶%多态性,单核苷酸%病理学,临床
乳腺腫瘤%胸苷痠閤酶%多態性,單覈苷痠%病理學,臨床
유선종류%흉감산합매%다태성,단핵감산%병이학,림상
Breast neoplasms%Thymidylate synthetase%Polymorphism,single-nucleotide%Pathology,clinical
目的 研究胸苷酸合成酶(thymidylate synthase,TS)基因多态性在汉族女性乳腺癌患者中的分布特点及其与临床病理之间的关系.方法收集经病理学确诊的83例初诊乳腺癌患者外周静脉血,术前均未给予任何针对乳腺癌的治疗,应用聚合酶链反应限制性片段长度多态性技术(PCR-RFLP)检测墓因型.基因多态性与临床病理的关系采用卡方分析进行检验.结果 83例乳腺癌患者的基因分布情况为:(1)3R/3R、2R/3R及2R/2R 3种基因型的频率分别为68.7%、27.7%、3.6%;(2)按G→C单核苷酸多态性细分,2R/2R,2R/3C,2R/3G,3G/3G,3C/3C及3G/3C 6种基因型的频率分别为3.6%、19.3%、8.4%、19.3%、37.3%及12.1%;(3)+/+6 bp、+/-6 bp及-/-6 bp3种基因型的频率分别为8.4%、50.6%及41.0%;(4)3种基因多态性均与乳腺癌的组织学分级相关(P＜0.05);另外TS 5′-UTR多态性与淋巴结转移(P=0.019)、Ki67(P=0.022)相关,TS 3′-UTR多态性与发病年龄相关(P=0.002),G→C单核苷酸多态性与淋巴结转移相关(P=0.021).结论 汉族女性乳腺癌患者中3R/3R、-/-6 bp基因型频率较高;TS基因多态性与乳腺癌临床病理因素密切相关.
目的 研究胸苷痠閤成酶(thymidylate synthase,TS)基因多態性在漢族女性乳腺癌患者中的分佈特點及其與臨床病理之間的關繫.方法收集經病理學確診的83例初診乳腺癌患者外週靜脈血,術前均未給予任何針對乳腺癌的治療,應用聚閤酶鏈反應限製性片段長度多態性技術(PCR-RFLP)檢測墓因型.基因多態性與臨床病理的關繫採用卡方分析進行檢驗.結果 83例乳腺癌患者的基因分佈情況為:(1)3R/3R、2R/3R及2R/2R 3種基因型的頻率分彆為68.7%、27.7%、3.6%;(2)按G→C單覈苷痠多態性細分,2R/2R,2R/3C,2R/3G,3G/3G,3C/3C及3G/3C 6種基因型的頻率分彆為3.6%、19.3%、8.4%、19.3%、37.3%及12.1%;(3)+/+6 bp、+/-6 bp及-/-6 bp3種基因型的頻率分彆為8.4%、50.6%及41.0%;(4)3種基因多態性均與乳腺癌的組織學分級相關(P＜0.05);另外TS 5′-UTR多態性與淋巴結轉移(P=0.019)、Ki67(P=0.022)相關,TS 3′-UTR多態性與髮病年齡相關(P=0.002),G→C單覈苷痠多態性與淋巴結轉移相關(P=0.021).結論 漢族女性乳腺癌患者中3R/3R、-/-6 bp基因型頻率較高;TS基因多態性與乳腺癌臨床病理因素密切相關.
목적 연구흉감산합성매(thymidylate synthase,TS)기인다태성재한족녀성유선암환자중적분포특점급기여림상병리지간적관계.방법수집경병이학학진적83례초진유선암환자외주정맥혈,술전균미급여임하침대유선암적치료,응용취합매련반응한제성편단장도다태성기술(PCR-RFLP)검측묘인형.기인다태성여림상병리적관계채용잡방분석진행검험.결과 83례유선암환자적기인분포정황위:(1)3R/3R、2R/3R급2R/2R 3충기인형적빈솔분별위68.7%、27.7%、3.6%;(2)안G→C단핵감산다태성세분,2R/2R,2R/3C,2R/3G,3G/3G,3C/3C급3G/3C 6충기인형적빈솔분별위3.6%、19.3%、8.4%、19.3%、37.3%급12.1%;(3)+/+6 bp、+/-6 bp급-/-6 bp3충기인형적빈솔분별위8.4%、50.6%급41.0%;(4)3충기인다태성균여유선암적조직학분급상관(P＜0.05);령외TS 5′-UTR다태성여림파결전이(P=0.019)、Ki67(P=0.022)상관,TS 3′-UTR다태성여발병년령상관(P=0.002),G→C단핵감산다태성여림파결전이상관(P=0.021).결론 한족녀성유선암환자중3R/3R、-/-6 bp기인형빈솔교고;TS기인다태성여유선암림상병리인소밀절상관.
Objective To investigate the distribution of thethymidylate synthase (TS) gene polymorphisms in Chinese female Han-nationality breast cancer patients, including tandem repeat polymorphisms in the enhancer region ( ER), 6 bp deletion/insertion (del6/ins6) polymorphism in the 3′untranslated region (3′-UTR) and G→C single-nucleotide polymorphism (SNP). The association of TS gene polymorphism with pathological results was retrospectively analyzed. Methods This study included 83 breast cancer patients who received no preoperative chemotherapy. The polymorphisms of TS, ER and TS 3′2UTR were determined by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. Results ( 1 ) Of the 83 patients, the frequencies of the TSER 3R/3 R, 2R/3 R and 2R/2R were 68.7％ ,27. 7％ and 3. 6％, respectively; (2) Due to existence of the G→C single nucleotide polymorphism,TSER genotypes were divided into 2R/2R, 2R/3C, 2R/3G, 3G/3G,3C/3C and 3G/3C,their frequencies were 3. 6％, 19. 3％, 8.4％, 19. 3％, 37. 3％ and 12. 1％, respectively; ( 3 ) Of the 83 patients, the frequencies of the TS 3′-UTR ins6/ias6、ins6/del6 and del6/del6 were 8.4％ 、50.6％ and 41.0％, respectively; (4) TSER polymorphisms were correlated with lymph node metastasis ( P = 0.019 ),Ki67 ( P = 0.022 ) and histological grade ( P = 0.042 ). The TS 3′-UTR polymorphisms were correlated with age ( P = 0.002) and histological grade (P = 0.042). G→C SNP were correlated with lymph node metastasis ( P = 0.021 ) and histological grade ( P = 0.028 ). Conclusions Chinese female Han-nationality breast cancer patients have more genotypes frequencies of the TSER 3R/3R and 3′-UTR del6/del6; The TS polymorphisms are correlated with pathological factors of breast cancer.
