CAJ | 학술논문

目的研究2个维吾尔族青少年发病的成人型糖尿病(MODY)家系成员血清代谢产物变化的特点.方法收集到2个新疆喀什地区维吾尔族四代健在的MODY家系,成员共52名,分析其成员一般资料及血糖、血脂、血压等指标,运用核磁共振氢谱(1H NMR)对各成员血清代谢产物进行检测,对采集图谱进行数据预处理后,采用主成分分析法(PCA)进行数据解读.根据血糖、血压、体重指数(BMI)高低各分两组对代谢产物进行比较.通过Pearson相关系数显著性差异检测及二维谱技术确定各组间血清中的差异性代谢成分.结果家系成员中糖尿病组与非糖尿病组血液代谢产物比较,异亮氨酸、酪氨酸含量降低,α-葡萄糖、β-葡萄糖含量增加,且差异均有统计学意义(均P＜0.05).家系成员中高血压组与正常血压组血液代谢产物比较,柠檬酸、肌醇、1-甲基组氨酸及酪氨酸含量均显著降低(均P＜0.05).正常BMI组与高BMI组血液代谢产物比较无显著差异.结论 MODY家系成员中糖尿病和高血压患者体内三羧酸循环代谢紊乱,反映糖尿病患者体内肝糖原分解和肌糖原酵解下降,而高血压患者体内脂肪代谢受阻.
목적 연구2개유오이족청소년발병적성인형당뇨병(MODY)가계성원혈청대사산물변화적특점.방법 수집도2개신강객십지구유오이족사대건재적MODY가계,성원공52명,분석기성원일반자료급혈당、혈지、혈압등지표,운용핵자공진경보(1H NMR)대각성원혈청대사산물진행검측,대채집도보진행수거예처리후,채용주성분분석법(PCA)진행수거해독.근거혈당、혈압、체중지수(BMI)고저각분량조대대사산물진행비교.통과Pearson상관계수현저성차이검측급이유보기술학정각조간혈청중적차이성대사성분.결과 가계성원중당뇨병조여비당뇨병조혈액대사산물비교,이량안산、락안산함량강저,α-포도당、β-포도당함량증가,차차이균유통계학의의(균P＜0.05).가계성원중고혈압조여정상혈압조혈액대사산물비교,저몽산、기순、1-갑기조안산급락안산함량균현저강저(균P＜0.05).정상BMI조여고BMI조혈액대사산물비교무현저차이.결론 MODY가계성원중당뇨병화고혈압환자체내삼최산순배대사문란,반영당뇨병환자체내간당원분해화기당원효해하강,이고혈압환자체내지방대사수조.
Objective To study the characteristics of serum metabolites in two Uyghur families with maturityonset diabetes of the young(MODY).Methods Two MODY families were composed of four generations of Uyghur with 52 members collected from Kashgar region,Xinjiang Uyghur Autonomous Region.The general information,blood glucose and lipid levels,and blood pressure were analyzed.Using 1H nuclear magnetic resonance (1H NMR )spectroscopy,serum metabolites were measured for each subject.After having conducted data pretreatment on the spectrogram,orthogonal partial least squares discriminant analysis ( OPLS-DA ) was used to interpret data.The subjects were divided into two groups according to blood glucose ( diabetes and non-diabetes ),blood pressure,body mass index ( BMI ) for comparing differences in the metabolites.The differences of serum metabolic components between two groups were determined using pearson correlation coefficients with significant difference detection and two-dimensional spectrum technology.Results lsoleucine and tyrosine levels in diabetes group were decreased significantly,while α-glucose and β-glucose levels were increased significantly compared with non-diabetes group( all P＜0.05 ).Citrate,phaseomannite,1 -methyl histidine,and tyrosine levels in hypertension group were all decreased significantly compared with normal blood pressure group( all P＜0.05 ).No significant metabonomic differences were observed between normal BMI group and high BMI group.Conclusions Metabonomic changes in diabetic patients from MODY families indicate that diabetic patients suffer from disordered tricarboxylic acid cycle ( TCA cycle )metabolism,with reduced glycolysis of glycogen in liver and muscle.There exist the metabolic disorder in TCA cycle and obstruction of fat metabolism in patients with hypertension from the MODY families.