CAJ | 학술논문

目的探讨D8/17抗原在风湿性心脏病(RHD)患者的表达情况,并对该抗原的特性进行初步研究.方法应用白种人制备的D8/17单克隆抗体,通过流式细胞术分析96例RHD患者和83例对照组患者B淋巴细胞中D8/17抗原的表达率,以表达率≥10％判为D8/17阳性.用免疫共沉淀法提取D8/17抗原,用串联质谱技术分析D8/17抗原的特性.结果 D8/17抗原在RHD患者和对照组患者B淋巴细胞中的表达率分别为(85.36±15.15)％和(82.89±14.55)％,两组间差异无统计学意义(P=0.436),且两组人群D8/17阳性率均为100％.D8/17抗原的相对分子质量约为40 000～67 000,最具可能匹配的蛋白是膜突蛋白和β-肌动蛋白.结论本研究未提示D8/17抗原与RHD相关；D8/17抗原最具可能匹配的蛋白是膜突蛋白或β-肌动蛋白.
목적 탐토D8/17항원재풍습성심장병(RHD)환자적표체정황,병대해항원적특성진행초보연구.방법 응용백충인제비적D8/17단극륭항체,통과류식세포술분석96례RHD환자화83례대조조환자B림파세포중D8/17항원적표체솔,이표체솔≥10％판위D8/17양성.용면역공침정법제취D8/17항원,용천련질보기술분석D8/17항원적특성.결과 D8/17항원재RHD환자화대조조환자B림파세포중적표체솔분별위(85.36±15.15)％화(82.89±14.55)％,량조간차이무통계학의의(P=0.436),차량조인군D8/17양성솔균위100％.D8/17항원적상대분자질량약위40 000～67 000,최구가능필배적단백시막돌단백화β-기동단백.결론 본연구미제시D8/17항원여RHD상관；D8/17항원최구가능필배적단백시막돌단백혹β-기동단백.
Objective To investigate the D8/17 antigen expression of patients with rheumatic heart disease (RHD) in Guangdong province and study the antigen's characteristics.Methods The level of D8/17 antigen expression on B lymphocytes was determined with flow cytometry assay in 96 RHD patients and 83 unaffected controls.The percentage of B-cells expressing the D8/17 antigen having more than 10％ was considered to be positive. D8/17 antigen was extracted by immunopreeipitation,and the antigen characteristics was analyzed by tandem mass spectrometry. Results The mean percentage of B-cells expressing the D8/17 antigen was (85.36 ± 15.15)％ in the RHD patients and (82.89 ±4.55)％ in the controls,with no significant difference between the two groups ( P =0.436).Moreover,the positive rate of the D8/17 cxprcssion was 100％ in either the RHD patients or the controls.The molecular weight of D8/17 antigen was found to be 40 000-67 000,and the purified protein was most likely to match moesin or β-actin.Conclusions B-cell antigen D8/17 is not associated with RHD in Guangdong province of China.Moesin or β-actin is the most likely protein to match D8/17 antigen.