中华全科医师杂志
中華全科醫師雜誌
중화전과의사잡지
CHINESE JOURNAL OF GENERAL PRACTITIONERS
2012年
3期
195-198
,共4页
邓星奇%邵金炎%顾薇%刘梅%程德山%余爱勇
鄧星奇%邵金炎%顧薇%劉梅%程德山%餘愛勇
산성기%소금염%고미%류매%정덕산%여애용
肺疾病,慢性阻塞性%白细胞介素32%白细胞介素8%肿瘤坏死因子α
肺疾病,慢性阻塞性%白細胞介素32%白細胞介素8%腫瘤壞死因子α
폐질병,만성조새성%백세포개소32%백세포개소8%종류배사인자α
Pulmonary disease,chronic obstructive%Interleukins-32%Interleukins-8%Tumor necrosis factor-alpha
目的 探讨IL-32在慢性阻塞性肺疾病(COPD)发病机制中的作用.方法 2010年10月至2011年5月,用ELISA法检测60例COPD急性加重期患者、60例稳定期患者及30例健康对照者的血清和诱导痰中IL-32和IL-8、肿瘤坏死因子α(TNF-α)水平,并分析IL-32水平与IL-8、TNF-α和气流阻塞之间的相关性.结果 COPD急性加重期患者血清IL-32含量[(175±88) ng/L]明显高于健康对照者[ (59 ±21) ng/L]和稳定期患者[(89±34) ng/L],均P＜0.05,COPD稳定期患者高于健康对照者(P＜0.05)；急性加重期患者的诱导痰IL-32含量[(163±117) ng/L]明显高于健康对照者[ (75±38) ng/L]和稳定期患者[(108±63) ng/L],均P＜0.05,稳定期患者高于健康对照者(P＜0.05).急性期诱导痰中IL-32水平与IL-8、TNF-α呈正相关(r值分别为0.49和0.53,均P＜0.01),与第一秒用力呼气容积( FEV1)占预计值百分比、FEV1/用力肺活量(FvC)和PaO2呈负相关(r=-0.44～ -0.33,均P＜0.01)；急性期血清中IL-32水平与IL-8、TNF-α呈正相关(r值分别为0.45和0.61,均P＜0.01),与FEV1占预计值百分比、FEV1/FVC和PaO2呈负相关(r值为-0.46 ～ -0.29,均P＜0.01).结论 IL-32参与COPD的炎症过程,此过程与IL-8、TNF-α等炎性因子有关.IL-32水平可能作为COPD急性发作的标志之一.
目的 探討IL-32在慢性阻塞性肺疾病(COPD)髮病機製中的作用.方法 2010年10月至2011年5月,用ELISA法檢測60例COPD急性加重期患者、60例穩定期患者及30例健康對照者的血清和誘導痰中IL-32和IL-8、腫瘤壞死因子α(TNF-α)水平,併分析IL-32水平與IL-8、TNF-α和氣流阻塞之間的相關性.結果 COPD急性加重期患者血清IL-32含量[(175±88) ng/L]明顯高于健康對照者[ (59 ±21) ng/L]和穩定期患者[(89±34) ng/L],均P＜0.05,COPD穩定期患者高于健康對照者(P＜0.05)；急性加重期患者的誘導痰IL-32含量[(163±117) ng/L]明顯高于健康對照者[ (75±38) ng/L]和穩定期患者[(108±63) ng/L],均P＜0.05,穩定期患者高于健康對照者(P＜0.05).急性期誘導痰中IL-32水平與IL-8、TNF-α呈正相關(r值分彆為0.49和0.53,均P＜0.01),與第一秒用力呼氣容積( FEV1)佔預計值百分比、FEV1/用力肺活量(FvC)和PaO2呈負相關(r=-0.44～ -0.33,均P＜0.01)；急性期血清中IL-32水平與IL-8、TNF-α呈正相關(r值分彆為0.45和0.61,均P＜0.01),與FEV1佔預計值百分比、FEV1/FVC和PaO2呈負相關(r值為-0.46 ～ -0.29,均P＜0.01).結論 IL-32參與COPD的炎癥過程,此過程與IL-8、TNF-α等炎性因子有關.IL-32水平可能作為COPD急性髮作的標誌之一.
목적 탐토IL-32재만성조새성폐질병(COPD)발병궤제중적작용.방법 2010년10월지2011년5월,용ELISA법검측60례COPD급성가중기환자、60례은정기환자급30례건강대조자적혈청화유도담중IL-32화IL-8、종류배사인자α(TNF-α)수평,병분석IL-32수평여IL-8、TNF-α화기류조새지간적상관성.결과 COPD급성가중기환자혈청IL-32함량[(175±88) ng/L]명현고우건강대조자[ (59 ±21) ng/L]화은정기환자[(89±34) ng/L],균P＜0.05,COPD은정기환자고우건강대조자(P＜0.05)；급성가중기환자적유도담IL-32함량[(163±117) ng/L]명현고우건강대조자[ (75±38) ng/L]화은정기환자[(108±63) ng/L],균P＜0.05,은정기환자고우건강대조자(P＜0.05).급성기유도담중IL-32수평여IL-8、TNF-α정정상관(r치분별위0.49화0.53,균P＜0.01),여제일초용력호기용적( FEV1)점예계치백분비、FEV1/용력폐활량(FvC)화PaO2정부상관(r=-0.44～ -0.33,균P＜0.01)；급성기혈청중IL-32수평여IL-8、TNF-α정정상관(r치분별위0.45화0.61,균P＜0.01),여FEV1점예계치백분비、FEV1/FVC화PaO2정부상관(r치위-0.46 ～ -0.29,균P＜0.01).결론 IL-32삼여COPD적염증과정,차과정여IL-8、TNF-α등염성인자유관.IL-32수평가능작위COPD급성발작적표지지일.
Objective To measure the levels of human interleukin (IL)-32 in the serum and induced sputum of patients with chronic obstructive pulmonary disease (COPD) and investigate the possible roles of IL-32 in COPD.Methods Sixty patients with acute exacerbation of COPD ( AECOPD),60 patients with stable COPD,and 30 healthy subjects were recruited.The concentrations of IL-8,tumor necrosis factor alpha (TNF-α),and IL-32 in serum and induced sputum were measured by enzyme-linked immunosorbent assay (ELISA).The correlations among IL-32,IL-8,TNF-α,and lung functions were investigated. The data were analyzed using a statistical software package (SPSS 13.0).Variables were compared with one-way ANOVA,and correlations among variables were analyzed using Pearson's correlation coefficient or Spearman's correlation coefficient.Results The serum IL-32 level was significantly higher in AECOPD patients [(175 ± 88) ng/L] than in healthy subjects [ (59 ± 21 ) ng/L] and in stable COPD patients [ (89 ± 34) ng/L] (P ＜ 0.05) ; the serum IL-32 level was also significantly higher in stable COPD patients than in healthy subjects (P ＜ 0.05).The sputum IL-32 level was significantly higher in AECOPD patients [ ( 163 ± 117) ng/L] than in healthy subjects [ ( 75 ± 38 ) ng/L] and stable COPD patients [ ( 108 ± 63 )ng/L] (P ＜0.05); the sputum IL-32 level was also significantly higher in stable COPD patients than in healthy subjects ( P ＜ 0.05 ).The sputum IL-32 level in AECOPD patients was positively correlated with the sputum IL-8 and TNF-α levels (r =0.49 and 0.53,respectively) (P ＜0.01 ).The sputum IL-32 level in AECOPD patients was negatively correlated with FEV1 predicted values,FEV1/FVC,and PaO2 (r =-0.44to -0.33) (P ＜ 0.01 ).The serum IL-32 level in AECOPD patients was positively correlated with the serum IL-8 and TNF-o levels (r =0.45 and 0.61,respectively) (P ＜ 0.01 ).The serum IL-32 level in AECOPD patients was negatively correlated with FEV1 predicted values,FEV1/FVC,and PaO2 (r =-0.46to - 0.29) ( P ＜ 0.01 ).Conclusions IL-32 may be involved in the pathogenesis of airway inflammation in COPD.IL-32 may be a useful marker of acute exacerbation of COPD.
