CAJ | 학술논문

目的探讨性激素对卵巢切除后大鼠膀胱结构和功能的作用.方法将70只成年雌性大鼠平均分成7组进行假手术和双侧卵巢切除.除假手术组和单纯卵巢切除组外分别给予苯甲酸雌二醇0.25 mg/kg、苯甲酸雌二醇1 mg/kg、黄体酮、苯甲酸雌二醇0.25 mg/kg和黄体酮、丙酸睾酮,隔日肌注,4周后处死.光镜和电镜下观察大鼠膀胱形态学变化.结果双侧卵巢切除后,膀胱壁变薄为(0.97±O.11)mm(P<0.05),雌激素和雄激素均可使膀胱重量和膀胱壁厚度明显增加,甚至超过假手术组(P<O.05).卵巢切除后膀胱逼尿肌肌间隙增大,肌细胞退变.雌激素和雄激素可以逆转此反应,高剂量的雌激素增生作用反而减弱,并使逼尿肌出现大量胞质空泡.孕激素对膀胱结构的影响不明显,但有削弱雌激素作用的趋向.结论体内雌激素和雄激素水平对膀胱结构有重要影响,两者均能促进膀胱逼尿肌增生.大剂量的雌激素可能损害肌细胞结构,孕激素可削弱雌激素对膀胱的作用.
목적 탐토성격소대란소절제후대서방광결구화공능적작용.방법 장70지성년자성대서평균분성7조진행가수술화쌍측란소절제.제가수술조화단순란소절제조외분별급여분갑산자이순0.25 mg/kg、분갑산자이순1 mg/kg、황체동、분갑산자이순0.25 mg/kg화황체동、병산고동,격일기주,4주후처사.광경화전경하관찰대서방광형태학변화.결과 쌍측란소절제후,방광벽변박위(0.97±O.11)mm(P<0.05),자격소화웅격소균가사방광중량화방광벽후도명현증가,심지초과가수술조(P<O.05).란소절제후방광핍뇨기기간극증대,기세포퇴변.자격소화웅격소가이역전차반응,고제량적자격소증생작용반이감약,병사핍뇨기출현대량포질공포.잉격소대방광결구적영향불명현,단유삭약자격소작용적추향.결론 체내자격소화웅격소수평대방광결구유중요영향,량자균능촉진방광핍뇨기증생.대제량적자격소가능손해기세포결구,잉격소가삭약자격소대방광적작용.
objective To investigate the effects of sex hormones on bladder and mechanism thereof.Methods Sixty mature female SD rats tinderwent bilateral ovariectomy and then randomly divided into 6 groups:low dose estrogen group(OVX+E group)treated with estradiol benzoate 0.25 mg/kg injected intramuscularly qod.high dose estrogen group(OVX+E 1 mg group)treated with estradiol benzoate 1 mg/kg,progesterone(P)group,treated with P 1 mg,OVX+E+P group treated with estradiol benzoate 0.25 mg/kg+progesterone 1 mg.d androgen group(OVX+T group)treated with testosterone propionate 3 mg/kg.and untreated ovariectomized group(OVX group)receiving no medication.Another 10 rats underwent sham operation.Four weeks later the rats were killed with their bladders and urethros taken out to undergo light and electron microscopy.Results The thickness of bladder wall of the OVX group was (O.97±0.11)mm,significant thinner than that of the sham operation group[(1.10±0.1)mm,P<O.05)].The thickness levels of bladder walls of the OVX+E and OVX+T groups were(1.23±0.12)mm and(1.25±0.12 mm)respectively.both significantly thicker than that of the OVX group(both P<0.01)Wider spaces between tlle detrusor muscle fascicles and degeneration of detrusor muscle cells were seen in the bladder walls of the OVX group.and such phenomena were milder in the OVX+E and OVX+T groups,however,wider spaces between the detrosor muscle fascicles could be seen in the OVX+E+P group too.The hypertrophic effect on the detrusor muscle of the OVX+E 1 mg group was weaker than that of the OVX+E group.a lot of cytoplasmic vacuoles was seen in the 2 groups treated with E,especially the OVX+E 1 mg group.The bladder structure of the OVX+P group was similar to that of the OVX group.Conclusion Endogenous testosterone level as well as estrogen level Can significantly affect the bladder structure wlth a hypertrophic effect on bladder detrusor muscle.But higher doze of estrogen also has some unexpected effects which may destroy bladder structure.In addition,progesterone can compromise the effect of estrogen.