中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2010年
3期
164-167
,共4页
王春燕%朱效娟%侯明%石艳%彭军%孙建芝%秦平%冀学斌%王琳%王谦
王春燕%硃效娟%侯明%石豔%彭軍%孫建芝%秦平%冀學斌%王琳%王謙
왕춘연%주효연%후명%석염%팽군%손건지%진평%기학빈%왕림%왕겸
地塞米松%B细胞活化因子%调节性T细胞%紫癜,血小板减少性
地塞米鬆%B細胞活化因子%調節性T細胞%紫癜,血小闆減少性
지새미송%B세포활화인자%조절성T세포%자전,혈소판감소성
Dexamethasone%B-cell activating factor%Regulatory T-cell%Purpura,thrombocy-topenic
目的 研究大剂量地塞米松治疗前后特发性血小板减少性紫瘕(ITP)患者B细胞活化因子(BAFF)和调节性T(Treg)细胞的变化.以及BAFF对Treg细胞的作用.方法 用ELISA方法 检测21例慢性活动性ITP患者治疗前后血浆BAFF水平;用流式细胞术检测患者治疗前后及外周血单个核细胞培养体系中Treg细胞的比例.结果 治疗前ITP患者的BAFF水平[(599.70±199.40)pg/ml]较正常对照[(454.50±132.50)pg/ml]明显升高(P<0.05),Treg细胞比例明显降低[(1.56±6.73)%](P<0.01);大剂量地塞米松治疗后,ITP患者的BAFF水平[(296.9±119.7)pg/ml]较治疗前明显下降(P<0.01),且低于正常对照组(P<0.01);Treg细胞比例明显升高[(5.94±2.22)%](P<0.01),且高于正常对照[(4.08±1.08)%](P<0.01).ITP患者BAFF水平和Treg比例与血小板计数无相关性(P>0.05).体外实验显示,rhBAFFO组和rhBAFF20组Treg数量无明显改变(P>0.05).结论 慢性ITP患者的BAFF水平增高,Treg细胞数量减少.大剂量地塞米松可降低患者的BAFF水平,增加Treg细胞的数量.BAFF对Treg细胞无明显影响.
目的 研究大劑量地塞米鬆治療前後特髮性血小闆減少性紫瘕(ITP)患者B細胞活化因子(BAFF)和調節性T(Treg)細胞的變化.以及BAFF對Treg細胞的作用.方法 用ELISA方法 檢測21例慢性活動性ITP患者治療前後血漿BAFF水平;用流式細胞術檢測患者治療前後及外週血單箇覈細胞培養體繫中Treg細胞的比例.結果 治療前ITP患者的BAFF水平[(599.70±199.40)pg/ml]較正常對照[(454.50±132.50)pg/ml]明顯升高(P<0.05),Treg細胞比例明顯降低[(1.56±6.73)%](P<0.01);大劑量地塞米鬆治療後,ITP患者的BAFF水平[(296.9±119.7)pg/ml]較治療前明顯下降(P<0.01),且低于正常對照組(P<0.01);Treg細胞比例明顯升高[(5.94±2.22)%](P<0.01),且高于正常對照[(4.08±1.08)%](P<0.01).ITP患者BAFF水平和Treg比例與血小闆計數無相關性(P>0.05).體外實驗顯示,rhBAFFO組和rhBAFF20組Treg數量無明顯改變(P>0.05).結論 慢性ITP患者的BAFF水平增高,Treg細胞數量減少.大劑量地塞米鬆可降低患者的BAFF水平,增加Treg細胞的數量.BAFF對Treg細胞無明顯影響.
목적 연구대제량지새미송치료전후특발성혈소판감소성자하(ITP)환자B세포활화인자(BAFF)화조절성T(Treg)세포적변화.이급BAFF대Treg세포적작용.방법 용ELISA방법 검측21례만성활동성ITP환자치료전후혈장BAFF수평;용류식세포술검측환자치료전후급외주혈단개핵세포배양체계중Treg세포적비례.결과 치료전ITP환자적BAFF수평[(599.70±199.40)pg/ml]교정상대조[(454.50±132.50)pg/ml]명현승고(P<0.05),Treg세포비례명현강저[(1.56±6.73)%](P<0.01);대제량지새미송치료후,ITP환자적BAFF수평[(296.9±119.7)pg/ml]교치료전명현하강(P<0.01),차저우정상대조조(P<0.01);Treg세포비례명현승고[(5.94±2.22)%](P<0.01),차고우정상대조[(4.08±1.08)%](P<0.01).ITP환자BAFF수평화Treg비례여혈소판계수무상관성(P>0.05).체외실험현시,rhBAFFO조화rhBAFF20조Treg수량무명현개변(P>0.05).결론 만성ITP환자적BAFF수평증고,Treg세포수량감소.대제량지새미송가강저환자적BAFF수평,증가Treg세포적수량.BAFF대Treg세포무명현영향.
Objective To investigate the change of B-cell activating factor of the TNF family (BAFF)and regulatory T-cells(Tregs)before and after high-dose dexamethasone(HD-DXM)therapy and assess the effect of BAFF on Treg cells in immune thrombocytopenic purpura(ITP).Methods The plasma BAFF concentration was measured by ELISA.and Treg cell numbers by flow cytometry.Results The plasma BAFF level[(599.70±199.40)pg/m1]was significantly increased(P<0.05),and the percentage ofTreg cells[(1.56±0.73)%]was significantly decreased(P<0.01)in ITP patients before treatment ascompared with that in controls[(454.5±132.5)pg/ml and(4.08±1.08)%,respectively].After treat-ment with HD-DXM,the plasma BAFF level[(296.9±119.7)PS/m1]was significantly decreased(P<0.01),and the percentage of Treg cells[(5.94±2.22)%]was significantly increased(P<0.01).The BAFF level and Treg proportion had no significant correlation with platelets count(P>0.05).In in vitroassays,no difference was found in the number of Treg cells between rhBAFF0 group and rhBAFF20 group[(1.53±0.69)%,(1.49 4-0.67)%,P=0.89)].Conclmion BAFF level was increased and Treg cellsdecreased in ITP patients.HD-DXM might play a role in ITP treatment by down-regulating BAFF expressionand up.regulating Treg cells number.BAFF had no influenee on the number of Treg cells.
