CAJ | 학술논문

目的：观察含血管活性肠肽(VIP)神经在爬行类动物-扬子鳄泄殖腔壁内的分布特点。方法：免疫组织化学ABC法及免疫荧光法。结果：扬子鳄泄殖腔壁内VIP免疫反应阳性神经纤维见于粘膜下层及肌层，呈串珠状，大致与平滑肌纤维平行走行，并见其分支在实质内构成网络状，但明显伴血管走行的纤维稀少。通过免疫荧光法证实，粘膜下层内存在VVIP免疫反应阳性细胞，胞体大小、形态不一。结论：扬子鳄泄殖腔已具备了类似高等脊椎动物的含VIP神经的调节。
목적：관찰함혈관활성장태(VIP)신경재파행류동물-양자악설식강벽내적분포특점。방법：면역조직화학ABC법급면역형광법。결과：양자악설식강벽내VIP면역반응양성신경섬유견우점막하층급기층，정천주상，대치여평활기섬유평행주행，병견기분지재실질내구성망락상，단명현반혈관주행적섬유희소。통과면역형광법증실，점막하층내존재VVIP면역반응양성세포，포체대소、형태불일。결론：양자악설식강이구비료유사고등척추동물적함VIP신경적조절。
Objective: To observe the distribution of nerves containing vasoactive intestinal peptide(VIP) in the reptile: cloaca wall of Alligator sinensis. Methods: Iummunohistochemical ABC and immunofluorescent methods were used. Results: Postive VIP-immunoreactive fibres in cloaca wall of Alligator sinensis were seen in submucosa and muscular larnina. They were characterized by their beaded appearance. They ran in general parallel to the smooth muscler fibers and some of their branches formed fiber neworks in substance. But the fibers that ran along blood vesels were rate.Immunofluorescent method showed that there were psotive VIP-immunoreactive cells in submucosa and the bodies were different in shape and size. Conclusion: The cloaca of ALligator sineniss also has regulation of nerves containing vasoactive intestinal peptide, which is similar to that of the organization of higher vertebrate.