CAJ | 학술논문

将家蚕核型多角体病毒（Bombyx mori Nuclear PolyhedrosisVirus，BmNPV）的解链酶基因DNA和苜蓿尺蠖核型多角体病毒（Autographa califonica Multiple Nuclear Polyhedrosis Virus，AcMNPV）的基因组DNA共转染到秋粘虫细胞系Sf-21，经过累代的筛选获得既能感染家蚕细胞，又能感染秋粘虫细胞的宿主域扩大的杂交型病毒HyNPV。
장가잠핵형다각체병독（Bombyx mori Nuclear PolyhedrosisVirus，BmNPV）적해련매기인DNA화목숙척확핵형다각체병독（Autographa califonica Multiple Nuclear Polyhedrosis Virus，AcMNPV）적기인조DNA공전염도추점충세포계Sf-21，경과루대적사선획득기능감염가잠세포，우능감염추점충세포적숙주역확대적잡교형병독HyNPV。
The helicase gene of Bombyx mori Nuclear Polyhedrosis Virus werecontransfected into Sf-21 cell with the genomic DNA of Autographa californica Nuclear Polyhedrosis Virus．The hostrange expanded hybrid virus （HyNPV），which could infect both Bm cell line and Sf cell line，was constructed via multiple generation selection．