中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2003年
17期
2402-2403
,共2页
激光%瘢痕%成纤维细胞%基因%凋亡
激光%瘢痕%成纖維細胞%基因%凋亡
격광%반흔%성섬유세포%기인%조망
laser%cicatrix%fibroblasts%gene%apoptosis
目的从蛋白质水平探讨氦氖激光诱导瘢痕成纤维细胞凋亡的机制.方法以 632.8 nm波长、 100 mW/cm2功率密度氦氖激光照射培养瘢痕成纤维细胞, 1次 /d, 30 min/次,连续照射 3 d,然后分别采用激光共聚焦扫描显微镜、流式细胞仪测定 bcl- 2,Fas,ICE蛋白的细胞分布与表达.结果在培养增生性瘢痕成纤维细胞有多种凋亡相关基因表达蛋白分布,氦氖激光照射后, Fas,ICE表达增加, bcl- 2表达降低.结论氦氖激光诱导的瘢痕成纤维细胞凋亡与 Fas,bcl- 2,ICE表达蛋白有关.
目的從蛋白質水平探討氦氖激光誘導瘢痕成纖維細胞凋亡的機製.方法以 632.8 nm波長、 100 mW/cm2功率密度氦氖激光照射培養瘢痕成纖維細胞, 1次 /d, 30 min/次,連續照射 3 d,然後分彆採用激光共聚焦掃描顯微鏡、流式細胞儀測定 bcl- 2,Fas,ICE蛋白的細胞分佈與錶達.結果在培養增生性瘢痕成纖維細胞有多種凋亡相關基因錶達蛋白分佈,氦氖激光照射後, Fas,ICE錶達增加, bcl- 2錶達降低.結論氦氖激光誘導的瘢痕成纖維細胞凋亡與 Fas,bcl- 2,ICE錶達蛋白有關.
목적종단백질수평탐토양내격광유도반흔성섬유세포조망적궤제.방법이 632.8 nm파장、 100 mW/cm2공솔밀도양내격광조사배양반흔성섬유세포, 1차 /d, 30 min/차,련속조사 3 d,연후분별채용격광공취초소묘현미경、류식세포의측정 bcl- 2,Fas,ICE단백적세포분포여표체.결과재배양증생성반흔성섬유세포유다충조망상관기인표체단백분포,양내격광조사후, Fas,ICE표체증가, bcl- 2표체강저.결론양내격광유도적반흔성섬유세포조망여 Fas,bcl- 2,ICE표체단백유관.
Aim To explore the mechanism of He- Ne laser inducing appotosis of fibroblasts of cultured hypertrophic scars(HS) at protein level. Methods The cultured fibroblasts in HS were irradiated with He- Ne laser (wavelength 632.8 nm, power density 100 mW/cm2) daily for 30 minutes. After 3 times of He- Ne laser irradiation, the expression of these genes such as bcl- 2, Fas and ICE were respectively studied by confocal laser scanning microscopy and flow cytometry.Results Several proteins such as bcl- 2,Fas and ICE proteins were presented in scar fibroblasts in culture.The amount of Fas and ICE proteins increased and bcl- 2 protein reduced after repeated He- Ne laser irradiation.Conclusion He- Ne laser inducing appotosis of scar fibroblasts is associated with these proteins of Fas,ICE and bcl- 2.
