CAJ | 학술논문

目的:从小花五味子中发现具有抗HIV活性的化学成分.方法:采用多种柱层析分离进行化合物的分离和纯化,通过波谱分析鉴定化合物的结构,化合物的抗HIV-1活性通过对HIV-1感染C8166细胞致细胞病变的抑制试验得到.结果:从小花五味子的茎藤部分分离得到了4个木脂素,分别鉴定为micrantherin A (1), gomisin K3 (2), gomisin G (3)和vladinol F (4).化合物4具有显著的抗HIV-1活性,IC50= 3.51 μg/mL,选择指数为27.45.结论:化合物1为新的木脂素,化合物4的抗HIV-1活性值得进一步研究.
목적:종소화오미자중발현구유항HIV활성적화학성분.방법:채용다충주층석분리진행화합물적분리화순화,통과파보분석감정화합물적결구,화합물적항HIV-1활성통과대HIV-1감염C8166세포치세포병변적억제시험득도.결과:종소화오미자적경등부분분리득도료4개목지소,분별감정위micrantherin A (1), gomisin K3 (2), gomisin G (3)화vladinol F (4).화합물4구유현저적항HIV-1활성,IC50= 3.51 μg/mL,선택지수위27.45.결론:화합물1위신적목지소,화합물4적항HIV-1활성치득진일보연구.
AIM: To find new natural compounds with anti-HIV activity from Schisandra micrantha. METHORD: The chemical constituents of Schisandra micrantha were isolated by various column chromatographic methods. Their structures were assigned by spectroscopic methods including 2D NMR experiments, and their anti-HIV-1 activities and cytotoxicities were tested by microtiter syncytium formation infectivity assay. RESULT: Four lignans, micrantherin A (1), gomisin K3 (2), gomisin G (3), and vladinol F (4) were isolated from the leaves and stems of Schisandra micrantha. Vladinol F (4) was found to show potent anti-HIV-1 activity with IC50 value of 3.51 μg/mL and a selective index of 27.45. CONCLUSION: Micrantherin A (1) was a new lignan.