神经科学通报(英文版)
神經科學通報(英文版)
신경과학통보(영문판)
NEUROSCIENCE BULLETIN
2008年
1期
29-33
,共5页
脊髓前角%5-羟色胺%色胺酸羟化酶%运动性疲劳%免疫组化
脊髓前角%5-羥色胺%色胺痠羥化酶%運動性疲勞%免疫組化
척수전각%5-간색알%색알산간화매%운동성피로%면역조화
ventral horn%spinal cord%5-hydroxytryptamine%tryptophan hydroxylase%exercise-induced fatigue%immunohistochemistry
目的 探讨运动性疲劳后脊髓前角5-羟色胺(5-hydroxytryptamine,5-HT)及其合成关键酶色胺酸羟化酶(tryptophan hydroxylase,TPH)表达的变化,阐释在脊髓水平运动性疲劳的发生机制.方法 健康成年雄性Wistar大鼠16只随机分为运动性疲劳组和对照组,采用免疫组化方法和计算机图像分析技术,检测大鼠脊髓前角的5-HT及TPH免疫反应阳性纤维及终末的平均积分光密度值.结果 运动性疲劳后,脊髓前角5-HT及TPH阳性反应纤维和终末减少,染色较浅,平均积分光密度值明显低于对照组(P＜0.05).结论 脊髓前角5-HT及TPH的含量在运动性疲劳后降低,提示脊髓前角5-HT与运动性疲劳的发生有关.
目的 探討運動性疲勞後脊髓前角5-羥色胺(5-hydroxytryptamine,5-HT)及其閤成關鍵酶色胺痠羥化酶(tryptophan hydroxylase,TPH)錶達的變化,闡釋在脊髓水平運動性疲勞的髮生機製.方法 健康成年雄性Wistar大鼠16隻隨機分為運動性疲勞組和對照組,採用免疫組化方法和計算機圖像分析技術,檢測大鼠脊髓前角的5-HT及TPH免疫反應暘性纖維及終末的平均積分光密度值.結果 運動性疲勞後,脊髓前角5-HT及TPH暘性反應纖維和終末減少,染色較淺,平均積分光密度值明顯低于對照組(P＜0.05).結論 脊髓前角5-HT及TPH的含量在運動性疲勞後降低,提示脊髓前角5-HT與運動性疲勞的髮生有關.
목적 탐토운동성피로후척수전각5-간색알(5-hydroxytryptamine,5-HT)급기합성관건매색알산간화매(tryptophan hydroxylase,TPH)표체적변화,천석재척수수평운동성피로적발생궤제.방법 건강성년웅성Wistar대서16지수궤분위운동성피로조화대조조,채용면역조화방법화계산궤도상분석기술,검측대서척수전각적5-HT급TPH면역반응양성섬유급종말적평균적분광밀도치.결과 운동성피로후,척수전각5-HT급TPH양성반응섬유화종말감소,염색교천,평균적분광밀도치명현저우대조조(P＜0.05).결론 척수전각5-HT급TPH적함량재운동성피로후강저,제시척수전각5-HT여운동성피로적발생유관.
Objective To investigate changes of 5-hydroxytryptamine (5-HT) and its synthesis rate-limiting enzyme tryptophan hydroxylase (TPH) in the ventral horn of spinal cord after exercise-induced fatigue, and to further discuss the mechanism of exercise-induced central fatigue at spinal level. Methods Sixteen healthy adult Wistar rats were randomly divided into 2 groups: exercise-induced fatigue group and control group. Immunohistochemical staining for 5-HT and TPH in the ventral horn were performed and analysized quantitatively. The mean optic densities of 5-HT and TPH positive fibers or terminals were measured by computerized image analyzer. Results Both 5-HT and TPH positive fibers/terminals decreased in the exercise-induced fatigue group. The immunohistochemical staining was weaker and the mean optic densities decreased obviously in the fatigue group compared with those in the control group (P ＜ 0.05). Conclusion 5-HT and TPH in the ventral horn of spinal cord might be involved in exercise-induced fatigue.
