CAJ | 학술논문

[目的]探讨可控微结构EBM钛合金支架作为成骨细胞体外培养载体的可行性,并观察载体类蜂巢状孔结构在成骨细胞培养中的作用.[方法]应用直接金属快速成形技术一电子束熔化成形(electron beam melting,EBM)过程直接构建和制备可控性微结构钛合金支架.分离培养1 d龄胎兔颅骨成骨细胞,以兔颅骨源性成骨细胞复合支架作为实验组,以单独培养的兔颅骨源性成骨细胞作为对照组.将成骨细胞与支架复合培养1、7、14 d后,进行倒置相差显微镜、扫描电镜及组织切片染色观察细胞与材料复合情况,以MTT比色法及碱性磷酸酶的定量检测研究材料结构对细胞增殖、分化的影响.[结果]成骨细胞/支架复合培养7d后,大量细胞通过伸出多个伪足粘附在支架表面以及孔周围并与支架牢固结合;培养14 d后支架表面及孔隙内有大量细胞分布,细胞伸出数个突起沿着孔隙壁逐渐向孔隙内延伸、汇集.支架上的细胞增殖、分化以及转移明显增加,与对照组比较有明显统计学差异(P<0.05).[结论]EBM钛合金支架有利于细胞的贴附、生长及增殖,并对细胞的功能无不良的影响.支架类蜂巢状孔结构能够调节成骨细胞在支架内的分布.
[목적]탐토가공미결구EBM태합금지가작위성골세포체외배양재체적가행성,병관찰재체류봉소상공결구재성골세포배양중적작용.[방법]응용직접금속쾌속성형기술일전자속용화성형(electron beam melting,EBM)과정직접구건화제비가공성미결구태합금지가.분리배양1 d령태토로골성골세포,이토로골원성성골세포복합지가작위실험조,이단독배양적토로골원성성골세포작위대조조.장성골세포여지가복합배양1、7、14 d후,진행도치상차현미경、소묘전경급조직절편염색관찰세포여재료복합정황,이MTT비색법급감성린산매적정량검측연구재료결구대세포증식、분화적영향.[결과]성골세포/지가복합배양7d후,대량세포통과신출다개위족점부재지가표면이급공주위병여지가뢰고결합;배양14 d후지가표면급공극내유대량세포분포,세포신출수개돌기연착공극벽축점향공극내연신、회집.지가상적세포증식、분화이급전이명현증가,여대조조비교유명현통계학차이(P<0.05).[결론]EBM태합금지가유리우세포적첩부、생장급증식,병대세포적공능무불량적영향.지가류봉소상공결구능구조절성골세포재지가내적분포.
[Objective]To explore the feasibility of culturing oateoblast on titanium alloy scaffold with controlled internal structure fabricated using electron beam melting techniques in vitre and to investigate the effects of like-honeycomb scaffold with coutrolled porous structure.[Method]A direct metal rapid prototyping(RP)fabrication technique,electron beam melting(EBM)process.was utilized to fabricate porous titanium alloy scaffold with fully interconnected and controlled internal pore structure.Osteoblasts were isolated from rabbit skulls,seeded on scaffolds,and cultured for up to 1,7 and 14 days respectively.The experiment was divided into experiment group(cells cultured with scaffold)and control group(cells alone).The growth of rabbit osteoblast on the scaffolds was observed by inverted phase contrast microscope,scanning electron microscope,and histological section staining method.Proliferation and differentiation of osteoblasts were determined at different time points by MTT assay and the activity of alkaline phoaphatase.[Result]The results showed the osteoblasts on scaffolds grew well in vitro with biological and morphological characteristics similar to those of normal osteoblasts.Good adheusion,proliferation and differentiation of the osteeblasts on the scaffold were noted with the culture time prolonged.The number of cells on scaffolds was higher than the control group(P<0.05).[Conclusion]The porous scaffold not only has good biocompatibility,but also improved the adhesion.growth and proliferation of osteoblasts,showing no adverse effects on the cell functions.The controlled like-honeycomb pore structure can adjust the distribution of osteoblasts on the scaffolds.