中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2010年
2期
277-281
,共5页
大鼠%PC12细胞%细胞凋亡%6-羟基多巴胺%c-Jun氨基末端激酶%神经生长因子
大鼠%PC12細胞%細胞凋亡%6-羥基多巴胺%c-Jun氨基末耑激酶%神經生長因子
대서%PC12세포%세포조망%6-간기다파알%c-Jun안기말단격매%신경생장인자
Rats%PC12 cells%Apoptosis%6-hydroxydopamine%c-Jun N-terminal kinase%Nerve growth factor
目的:以6-羟基多巴胺(6-OHDA)作用于大鼠肾上腺嗜铬细胞瘤细胞(PC12 cells)以诱导其凋亡,然后在其中分别加入神经生长因子(NGF)及c-Jun氨基端激酶(JNK)阻断剂SP600125,研究在加入NGF后JNK的活性与凋亡的关系.方法:实验分为对照组、6-OHDA组、NGF组、6-OHDA+NGF组、6-OHDA+JNK阻断剂SP600125组,以流式细胞分析法检测各组PC12细胞的凋亡率,以免疫印迹(Western blotting)法检测各组PC12细胞JNK的活化情况.结果:6-OHDA导致PC12细胞凋亡,JNK1活性提高;预孵SP600125或NGF15min后再加入6-OHDA则PC12细胞凋亡率及JNK1活性均降低.结论:JNK1参与了6-OHDA致PC12细胞凋亡作用,NGF抗6-OHDA所诱导的PC12细胞凋亡作用与其抑制JNK的活化有关.
目的:以6-羥基多巴胺(6-OHDA)作用于大鼠腎上腺嗜鉻細胞瘤細胞(PC12 cells)以誘導其凋亡,然後在其中分彆加入神經生長因子(NGF)及c-Jun氨基耑激酶(JNK)阻斷劑SP600125,研究在加入NGF後JNK的活性與凋亡的關繫.方法:實驗分為對照組、6-OHDA組、NGF組、6-OHDA+NGF組、6-OHDA+JNK阻斷劑SP600125組,以流式細胞分析法檢測各組PC12細胞的凋亡率,以免疫印跡(Western blotting)法檢測各組PC12細胞JNK的活化情況.結果:6-OHDA導緻PC12細胞凋亡,JNK1活性提高;預孵SP600125或NGF15min後再加入6-OHDA則PC12細胞凋亡率及JNK1活性均降低.結論:JNK1參與瞭6-OHDA緻PC12細胞凋亡作用,NGF抗6-OHDA所誘導的PC12細胞凋亡作用與其抑製JNK的活化有關.
목적:이6-간기다파알(6-OHDA)작용우대서신상선기락세포류세포(PC12 cells)이유도기조망,연후재기중분별가입신경생장인자(NGF)급c-Jun안기단격매(JNK)조단제SP600125,연구재가입NGF후JNK적활성여조망적관계.방법:실험분위대조조、6-OHDA조、NGF조、6-OHDA+NGF조、6-OHDA+JNK조단제SP600125조,이류식세포분석법검측각조PC12세포적조망솔,이면역인적(Western blotting)법검측각조PC12세포JNK적활화정황.결과:6-OHDA도치PC12세포조망,JNK1활성제고;예부SP600125혹NGF15min후재가입6-OHDA칙PC12세포조망솔급JNK1활성균강저.결론:JNK1삼여료6-OHDA치PC12세포조망작용,NGF항6-OHDA소유도적PC12세포조망작용여기억제JNK적활화유관.
AIM: To investigate the anti-apoptotic effect of nerve growth factor (NGF) on PC12 cells and to observe the mechanism of signal transduction of JNK pathway. METHODS: PC12 cells were treated with 6-hydroxydopamine (6-OHDA) to induce cell apoptosis. NGF and SP600125, the c-Jun N-terminal kinase inhibitors, were added respectively in order to study the relationship between the activity of c-Jun N-terminal kinase and apoptosis of PC12 cells. The cells were divided into control group, 6- OHDA group, NGF group, 6-OHDA plus NGF group, NGF plus SP600125 group. The apoptotic rates of PC12 cells with different treatments were detected by flow cytometry and activities of JNK in PC12 cells were determined by Western blotting. RESULTS: 6-OHDA increased the apoptotic rate and activity of JNK1 in PC12 cells. Incubation with SP600125 or NGF for 15 min before adding 6-OHDA decreased the apoptotic rate of PC12 cells and activity of JNK1 in PC12 cells.CONCLUSION: Activity of JNK is involved in the pro-apoptotic effect of 6-OHDA on PC12 cells. Anti-apoptotic effect of NGF on PC12 cells affected by 6-OHDA is related to the decrease in the activity of JNK1.
