CAJ | 학술논문

[目的]研究北冰洋产淀粉酶海洋细菌的筛选与鉴定及其产酶条件的优化.[方法]从156株北冰洋海洋细菌中筛选出淀粉酶高产菌株ArcB84A,并对其进行了菌株形态学鉴定、16S rRNA分子鉴定以及产酶条件优化.[结果]菌株ArcB84A属于交替假单胞菌属.菌株B84A的最佳产酶条件为:培养基起始pH值为7.0-8.0;最佳碳源为5‰葡萄糖;最佳氮源为5‰蛋白胨;TritonX-100、Tween20、Tween80等表面活性剂可以提高菌株淀粉酶活性,其中10‰ Tween80的效果最为明显.[结论]该研究为海洋细菌淀粉酶的生产与应用提供了理论依据.
[목적]연구북빙양산정분매해양세균적사선여감정급기산매조건적우화.[방법]종156주북빙양해양세균중사선출정분매고산균주ArcB84A,병대기진행료균주형태학감정、16S rRNA분자감정이급산매조건우화.[결과]균주ArcB84A속우교체가단포균속.균주B84A적최가산매조건위:배양기기시pH치위7.0-8.0;최가탄원위5‰포도당;최가담원위5‰단백동;TritonX-100、Tween20、Tween80등표면활성제가이제고균주정분매활성,기중10‰ Tween80적효과최위명현.[결론]해연구위해양세균정분매적생산여응용제공료이론의거.
[Objective] The aims were to investigate the screening and identification of amylase-producing marine bacteria from Arctic sea and the optimization of the amylase producing conditions. [Method] A high-yield strain for producing amylase named ArcB84A was isolated from a total of 156 marine bacteria of Arctic sea. Then, the morphological identification of the strain, molecular identification of 16S rRNA and optimization of fermentation conditions were conducted. [Result] ArcB84A strain was a member of Pseudoalteromonas genus. The optimum conditions for enzyme production of B84A strain included that, the initial pH value of the medium was 7.0-8.0, and the best carbon and nitrogen sources respectively were 5‰ glucose and peptone. Surfactants including TritonX-100, Tween20 and Tween80 could increase amylase activity of the strain, in which, the effect of 10‰ Tween80 was the most obvious.