CAJ | 학술논문

为使马铃薯试管薯工厂化生产,对试管薯生产参试进行了优化。试验结果表明：在2MS培养基中壮苗,是获得试管薯高产的必要前提。在诱导培养基MS＋6BA0.5 mg/L＋8%白糖液体培养基上黑暗处理2周后放入自然光下培养可提高试管薯的产量。当容器使用220 mL培养瓶时,诱导培养液量为50 mL为宜,并且每瓶放入20个茎段效果最好。试管苗去掉顶部后进行试管薯生产可提高成苗率和试管薯产量。
위사마령서시관서공엄화생산,대시관서생산삼시진행료우화。시험결과표명：재2MS배양기중장묘,시획득시관서고산적필요전제。재유도배양기MS＋6BA0.5 mg/L＋8%백당액체배양기상흑암처리2주후방입자연광하배양가제고시관서적산량。당용기사용220 mL배양병시,유도배양액량위50 mL위의,병차매병방입20개경단효과최호。시관묘거도정부후진행시관서생산가제고성묘솔화시관서산량。
In order to carry on the bunch production of microtubers,the optimization was made for the production parameters.Using 2 times of MS medium was a necessary premise for achieving robust plantlets in vitro and thereby a high yield of microtubers.Yield of microtubers was increased when plantlets in vitro were cultured in the inducing liquid medium MS ＋ 6 BA 0.5 mg / L ＋ 8% white sugar in the dark for two weeks and then under natural light.The best effects were achieved when 50 mL of inducing medium were added into 220 mL wide mouth bottle and 20 segments were inoculated.Furthermore,the plantlet in vitro survival percentage and microtuber yield were improved when the top segment was abandoned and other segments were kept for initiating the culture.