国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2012年
1期
24-26
,共3页
刘芳%李一苇%李晓寒%刘启林%陈仲全
劉芳%李一葦%李曉寒%劉啟林%陳仲全
류방%리일위%리효한%류계림%진중전
手足口病%病原学%EV71%实时荧光定量PCR
手足口病%病原學%EV71%實時熒光定量PCR
수족구병%병원학%EV71%실시형광정량PCR
Hand,foot and mouth disease%Etiology%EV71%Real-time PCR
目的 明确2011年引起菏泽市手足口病(HFMD)的病原体型别及分布特征.方法 采集临床诊断为手足口病病例的粪便或咽拭子样本,用Real-time PCR方法进行肠道病毒通用(EV)型、EV71型、CoxA16型的核酸检测,并对结果进行分析.结果 389例HFMD临床诊断病例样本检测,肠道病毒核酸阳性376例,检出率96.66％.其中EV71型阳性343例,占阳性病例总数的91.22％.核酸阳性病例分布在各县区,以牡丹区和定陶县最多,男女性别比例为2.69∶1,以3岁以下婴幼儿最多(88.56％).结论 2011年菏泽市手足口病病原体主要是EV71型肠道病毒.
目的 明確2011年引起菏澤市手足口病(HFMD)的病原體型彆及分佈特徵.方法 採集臨床診斷為手足口病病例的糞便或嚥拭子樣本,用Real-time PCR方法進行腸道病毒通用(EV)型、EV71型、CoxA16型的覈痠檢測,併對結果進行分析.結果 389例HFMD臨床診斷病例樣本檢測,腸道病毒覈痠暘性376例,檢齣率96.66％.其中EV71型暘性343例,佔暘性病例總數的91.22％.覈痠暘性病例分佈在各縣區,以牡丹區和定陶縣最多,男女性彆比例為2.69∶1,以3歲以下嬰幼兒最多(88.56％).結論 2011年菏澤市手足口病病原體主要是EV71型腸道病毒.
목적 명학2011년인기하택시수족구병(HFMD)적병원체형별급분포특정.방법 채집림상진단위수족구병병례적분편혹인식자양본,용Real-time PCR방법진행장도병독통용(EV)형、EV71형、CoxA16형적핵산검측,병대결과진행분석.결과 389례HFMD림상진단병례양본검측,장도병독핵산양성376례,검출솔96.66％.기중EV71형양성343례,점양성병례총수적91.22％.핵산양성병례분포재각현구,이모단구화정도현최다,남녀성별비례위2.69∶1,이3세이하영유인최다(88.56％).결론 2011년하택시수족구병병원체주요시EV71형장도병독.
Objective To study the type and distribution of the pathogen of HFMD in Heze city 2011.Methods Stool or throat swab specimens of clinically diagnosed cases with HFMD were collected,and Enterovirus (EV),Enterovirus 71 ( EV71 ) and Coxsackievirus A16 (CoxA16) nucleic acid examination was done by Real-time PCR.Results 389 cases were tested,and 376(96.66％ ) cases were Enterovirus-positive,including 343 (91.22％) cases of EV71 positive. Confirmed cases were in 9 districts or counties ; most cases were in Mudan area and Dingtao county.The ratio of male to female was 2.69 ∶ 1.88.56％ of confirmed cases were less than 3 years old.Conclusion The HFMD in Heze city was mainly caused by EV71.
