CAJ | 학술논문

目的研究乳腺浸润性导管癌组织中CD24的表达及与细胞核增殖抗Ki67及凋亡抑制蛋白Bcl-2的关系,探讨CD24在乳腺癌发生发展中的作用.方法应用免疫组化ElivisiDnTM Plus二步法检测86例乳腺浸润性导管癌组织和30例非瘤乳腺上皮组织中CD24及Ki67、Bcl-2的表达情况,分析CD24表达与Ki67、Bcl-2以及患者年龄、肿瘤大小、组织病理学分级、淋巴结转移、临床分期等临床病理学参数之间的关系.结果在86例乳腺浸润性导管癌中CD24表达的阳性率为87.2%(75/86),与对照组比较均有统计学差异(P<0.01);CD24表达与患者年龄、肿瘤大小、组织病理学分级、临床分期未见显著相关(P0.05),与腋窝淋巴结转移呈正相关(P<0.01);乳腺癌CD24表达与Ki67表达呈正相关(P<0.01),与Bel-2表达没有相关性(P0.05).结论 CIY24蛋白的表达与乳腺癌细胞增殖及侵袭转移具有相关性,是判断肿瘤生物学行为和患者预后的重要参考指标之一.
목적 연구유선침윤성도관암조직중CD24적표체급여세포핵증식항Ki67급조망억제단백Bcl-2적관계,탐토CD24재유선암발생발전중적작용.방법 응용면역조화ElivisiDnTM Plus이보법검측86례유선침윤성도관암조직화30례비류유선상피조직중CD24급Ki67、Bcl-2적표체정황,분석CD24표체여Ki67、Bcl-2이급환자년령、종류대소、조직병이학분급、림파결전이、림상분기등림상병이학삼수지간적관계.결과 재86례유선침윤성도관암중CD24표체적양성솔위87.2%(75/86),여대조조비교균유통계학차이(P<0.01);CD24표체여환자년령、종류대소、조직병이학분급、림상분기미견현저상관(P0.05),여액와림파결전이정정상관(P<0.01);유선암CD24표체여Ki67표체정정상관(P<0.01),여Bel-2표체몰유상관성(P0.05).결론 CIY24단백적표체여유선암세포증식급침습전이구유상관성,시판단종류생물학행위화환자예후적중요삼고지표지일.
Objective To investigate the expression of CD24 and Ki67 in the invasive breast ductal car-cinoma tissues, so as to assess the role of CD24 protein in the carcinogenesis and progression of breast canc-er. Methods The expression of CD24 and Ki67, Bcl-2 was examined by immunohistochemically in 86 breast cancer specimens and 30 non-cancer specimens ( ElivisionTM Plus). The relationship between CD24 expression and Ki67, Bcl-2, patient age, tumor size, histopathologic grade, axillary lymphnode metastasis, and clinical stage of breast cancer was analyzed. Results In 86 eases of invasive breast ductal carcinoma, the positive incidences of CD24 was 87.2% (75/86), which distinguished apparently from those of the con-trol group( P <0.01 ). In breast carcinoma tissues, the expression of CD24 was positively related to axillary lymphnode metastasis(P <0.01 ), but was not related to patients age,tumor size, histopathologic grade and clinical stage(P>0.05). CD24 expression was positively related to Ki67 (P<0.01 ) and negatively relat-ed to Bcl-2(P>0.05). Conclusion CD24 expression was related to the proliferation, invasion and me-tastasis of breast cancer cells and it can serve as an important marker for predicting biological behavior and prognosis of tumors.