中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2012年
1期
19-21
,共3页
刘涛%王统玲%勾善淼%殷涛%汪理%周伟%李永峰%王春友
劉濤%王統玲%勾善淼%慇濤%汪理%週偉%李永峰%王春友
류도%왕통령%구선묘%은도%왕리%주위%리영봉%왕춘우
胰腺肿瘤%胰岛素%增殖%侵袭%微环境
胰腺腫瘤%胰島素%增殖%侵襲%微環境
이선종류%이도소%증식%침습%미배경
Pancreatic neoplasms%Insulin%Proliferation%Invasion%Microenvironment
目的 研究胰岛素对人胰腺癌PANC1细胞增殖、侵袭能力及细胞低氧诱导因子(HIF-1α)、血管内皮生长因子( VEGF)表达的影响.方法 采用0.1、1、10、100 nmol/L胰岛素处理PANC1.噻唑蓝法和Transwell小室侵袭实验检测细胞增殖和侵袭能力；蛋白质印迹法和实时PCR法检测增殖性细胞核抗原(PCNA)及HIF-1α、VEGF蛋白和mRNA的表达.结果 胰岛素呈剂量依赖性诱导PANC1细胞的增殖,上调HIF-1α、VEGF蛋白表达.100 nmol/L胰岛素干预4d,PANC1细胞的PCNA蛋白表达显著上调(1.196±0.014比1.157±0.013,P＜0.05)；Transwell小室穿膜细胞数量显著增加[(141.0±2.1)个比(89.0±1.4)个,P＜0.05]；HIF-1α蛋白表达显著上调(1.139±0.020比0.598 ±0.013,P＜0.05),但HIF-1α mRNA表达无明显变化；VEGF蛋白表达显著上调(1.011±0.023比0.627±0.013,P ＞0.05),VEGF mRNA表达亦显著上调(0.970±0.016比0.350±0.013,P＜0.05).结论 高胰岛素微环境可诱导PANC1细胞增殖,并通过上调HIF-1α及VEGF的表达增强细胞的侵袭能力.
目的 研究胰島素對人胰腺癌PANC1細胞增殖、侵襲能力及細胞低氧誘導因子(HIF-1α)、血管內皮生長因子( VEGF)錶達的影響.方法 採用0.1、1、10、100 nmol/L胰島素處理PANC1.噻唑藍法和Transwell小室侵襲實驗檢測細胞增殖和侵襲能力；蛋白質印跡法和實時PCR法檢測增殖性細胞覈抗原(PCNA)及HIF-1α、VEGF蛋白和mRNA的錶達.結果 胰島素呈劑量依賴性誘導PANC1細胞的增殖,上調HIF-1α、VEGF蛋白錶達.100 nmol/L胰島素榦預4d,PANC1細胞的PCNA蛋白錶達顯著上調(1.196±0.014比1.157±0.013,P＜0.05)；Transwell小室穿膜細胞數量顯著增加[(141.0±2.1)箇比(89.0±1.4)箇,P＜0.05]；HIF-1α蛋白錶達顯著上調(1.139±0.020比0.598 ±0.013,P＜0.05),但HIF-1α mRNA錶達無明顯變化；VEGF蛋白錶達顯著上調(1.011±0.023比0.627±0.013,P ＞0.05),VEGF mRNA錶達亦顯著上調(0.970±0.016比0.350±0.013,P＜0.05).結論 高胰島素微環境可誘導PANC1細胞增殖,併通過上調HIF-1α及VEGF的錶達增彊細胞的侵襲能力.
목적 연구이도소대인이선암PANC1세포증식、침습능력급세포저양유도인자(HIF-1α)、혈관내피생장인자( VEGF)표체적영향.방법 채용0.1、1、10、100 nmol/L이도소처리PANC1.새서람법화Transwell소실침습실험검측세포증식화침습능력；단백질인적법화실시PCR법검측증식성세포핵항원(PCNA)급HIF-1α、VEGF단백화mRNA적표체.결과 이도소정제량의뢰성유도PANC1세포적증식,상조HIF-1α、VEGF단백표체.100 nmol/L이도소간예4d,PANC1세포적PCNA단백표체현저상조(1.196±0.014비1.157±0.013,P＜0.05)；Transwell소실천막세포수량현저증가[(141.0±2.1)개비(89.0±1.4)개,P＜0.05]；HIF-1α단백표체현저상조(1.139±0.020비0.598 ±0.013,P＜0.05),단HIF-1α mRNA표체무명현변화；VEGF단백표체현저상조(1.011±0.023비0.627±0.013,P ＞0.05),VEGF mRNA표체역현저상조(0.970±0.016비0.350±0.013,P＜0.05).결론 고이도소미배경가유도PANC1세포증식,병통과상조HIF-1α급VEGF적표체증강세포적침습능력.
Objective To investigate the effects of insulin on the proliferation and invasion of human pancreatic cancer cells PANC1,and on its HIF-1α,VEGF expression.Methods PANC1 was pretreated with insulin of different concentrations (0.1,1,10,100 nmol/L).The proliferation of PANC1 was tested by MTTmethod,and transwell assay was used to test the invasion ability of PANC1.HIF-1α,VEGF and PCNA protein expression was assessed by Western blots,and HIF-1α,VEGF mRNA was detected by real-time PCR.Results Insulin could increase the proliferation of PANC1 in a dose-dependent manner (p ＜0.05 ),and increase the expression of HIF-1α,VEGF protein.After 100 nmol/L insulin treatment for4 d,the PCNA protein expression in the insulin group was significantly higher than that in the control group (1.196 ±0.014 vs 1.157 ±0.013,P ＜ 0.05).The cancer cells passed through the chamber in insulin group were much more than that in the control group ( 141.0 ± 2.1 vs 89.0 ± 1.4,P ＜0.05 ).The expression of HIF-1α protein was significantly increased (1.139 ±0.020 vs 0.598 ±0.013,P ＜0.05),while there was no significant change of HIF-1αmRNA expression.Both the expression of VEGF protein and mRNA were significantly increased (1.011 ± 0.023 vs 0.627 ± 0.013 0.970 ± 0.016 vs 0.350 ± 0.01 3,P ＜ 0.05 ).Conclusions High insulin microenvironment could enhance the proliferation and invasion of PANC1 cells by up-regulating the expression of HIF-1α and VEGF.
