中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
7期
522-524
,共3页
孙迪%范雪云%王良群%李翠兰
孫迪%範雪雲%王良群%李翠蘭
손적%범설운%왕량군%리취란
矽肺%白细胞介素-8%多态性,单核苷酸%易感性
矽肺%白細胞介素-8%多態性,單覈苷痠%易感性
석폐%백세포개소-8%다태성,단핵감산%역감성
Silicosis%Interleukin-8%Polymorphism,single nucleotide%Disease susceptibility
目的 探讨白细胞介素(IL)-8基因多态性与矽肺易感性的关系.方法 选择确诊的101例矽肺患者为病例组,以接触同性质粉尘、首次诊断年龄相近的非矽肺的接尘工人为对照(121例).采集外周静脉血,盐析法提取DNA,应用聚合酶链-限制性片段长度多态性(PCR-RFLP)技术检测IL-8(Met31Arg、781C/T、-251A/T、RA+860)4个位点的基因型和等位基因频率并进行分析.结果 两组对象的首次诊断年龄、累积接尘工龄、吸烟率的差异均无统计学意义(P＞0.10).病例组IL-8(Met31Arg)GT基因型的分布频率为12.87%,对照组为2.48%,差异有统计学意义(P＜0.05),病例组和对照组携带等位基因G的频率分别为6.44%和2.07%,差异有统计学意义(P＜0.05).病例组IL-8(-251A/T)从基因型的分布频率为9.90%,对照组为25.62%,差异有统计学意义(P＜0.05).病例组IL-8(781C/T)基因型CC、CT、TT的分布频率分别为38.61%、40.59%、20.79%,与对照组(46.28%、40.50%、13.22%)相比,差异无统计学意义(P＞0.05).病例组IL-8(RA+860)基因型GG、GC、CC的分布频率分别为75.25%、21.78%、2.97%,与对照组(80.17%、14.88%、4.96%)相比,差异无统计学意义(P＞0.05).结论 IL-8的Met31Arg位点和一251MT位点的基因多态性与矽肺易感性有关,携带IL-8(Met31Arg)GT基因型的接尘工人患矽肺的危险性增加;携带IL-8(-251)从基因型的接尘工人患矽肺的危险性降低.未发现IL-8(781C/T和RA+860)位点的基因多态性与矽肺易感性有关.
目的 探討白細胞介素(IL)-8基因多態性與矽肺易感性的關繫.方法 選擇確診的101例矽肺患者為病例組,以接觸同性質粉塵、首次診斷年齡相近的非矽肺的接塵工人為對照(121例).採集外週靜脈血,鹽析法提取DNA,應用聚閤酶鏈-限製性片段長度多態性(PCR-RFLP)技術檢測IL-8(Met31Arg、781C/T、-251A/T、RA+860)4箇位點的基因型和等位基因頻率併進行分析.結果 兩組對象的首次診斷年齡、纍積接塵工齡、吸煙率的差異均無統計學意義(P＞0.10).病例組IL-8(Met31Arg)GT基因型的分佈頻率為12.87%,對照組為2.48%,差異有統計學意義(P＜0.05),病例組和對照組攜帶等位基因G的頻率分彆為6.44%和2.07%,差異有統計學意義(P＜0.05).病例組IL-8(-251A/T)從基因型的分佈頻率為9.90%,對照組為25.62%,差異有統計學意義(P＜0.05).病例組IL-8(781C/T)基因型CC、CT、TT的分佈頻率分彆為38.61%、40.59%、20.79%,與對照組(46.28%、40.50%、13.22%)相比,差異無統計學意義(P＞0.05).病例組IL-8(RA+860)基因型GG、GC、CC的分佈頻率分彆為75.25%、21.78%、2.97%,與對照組(80.17%、14.88%、4.96%)相比,差異無統計學意義(P＞0.05).結論 IL-8的Met31Arg位點和一251MT位點的基因多態性與矽肺易感性有關,攜帶IL-8(Met31Arg)GT基因型的接塵工人患矽肺的危險性增加;攜帶IL-8(-251)從基因型的接塵工人患矽肺的危險性降低.未髮現IL-8(781C/T和RA+860)位點的基因多態性與矽肺易感性有關.
목적 탐토백세포개소(IL)-8기인다태성여석폐역감성적관계.방법 선택학진적101례석폐환자위병례조,이접촉동성질분진、수차진단년령상근적비석폐적접진공인위대조(121례).채집외주정맥혈,염석법제취DNA,응용취합매련-한제성편단장도다태성(PCR-RFLP)기술검측IL-8(Met31Arg、781C/T、-251A/T、RA+860)4개위점적기인형화등위기인빈솔병진행분석.결과 량조대상적수차진단년령、루적접진공령、흡연솔적차이균무통계학의의(P＞0.10).병례조IL-8(Met31Arg)GT기인형적분포빈솔위12.87%,대조조위2.48%,차이유통계학의의(P＜0.05),병례조화대조조휴대등위기인G적빈솔분별위6.44%화2.07%,차이유통계학의의(P＜0.05).병례조IL-8(-251A/T)종기인형적분포빈솔위9.90%,대조조위25.62%,차이유통계학의의(P＜0.05).병례조IL-8(781C/T)기인형CC、CT、TT적분포빈솔분별위38.61%、40.59%、20.79%,여대조조(46.28%、40.50%、13.22%)상비,차이무통계학의의(P＞0.05).병례조IL-8(RA+860)기인형GG、GC、CC적분포빈솔분별위75.25%、21.78%、2.97%,여대조조(80.17%、14.88%、4.96%)상비,차이무통계학의의(P＞0.05).결론 IL-8적Met31Arg위점화일251MT위점적기인다태성여석폐역감성유관,휴대IL-8(Met31Arg)GT기인형적접진공인환석폐적위험성증가;휴대IL-8(-251)종기인형적접진공인환석폐적위험성강저.미발현IL-8(781C/T화RA+860)위점적기인다태성여석폐역감성유관.
Objective To explore the relationship between the polymorphisms of interleukin-8(IL-8)and the silicosis susceptibility. Methods The case group consisted of 101 male patients with stage I silicosis diagnosed by the Pneumoconiosis Diagnosis Expert Panel according to the Chinese National Diagnosis Criteria of Pneumoconiosis (GBZ 70-2009). The control group consisted of 121 workers without silicosis exposed to same dusts. The cases and the controls had the same dust exposure history. The peripheral venous blood was drawn from each subject. DNA was extracted from leucocytes by the salting method. The polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) techniques and PCR were used to examine polymorphism of IL-8(Met31Arg, 781C/T,-251A/T and RA+860). Results There were no the differences of age, cumulative exposure time and smoking between the cases and the controls (P＞0.05). The frequencies of IL-8 (Met31Arg) GT genotypes in cases and controls were 12.87% and 2.48%, respectively, there was significant difference (P＜0.05).The frequencies of allele G in cases and controls were 6.44% and 2.07%, respectively, there was significant difference (P＜0.05). The frequencies of IL-8 (-251A/T) AA genotypes in cases and controls were 9.90% and 25.64%, respectively, there was significant difference (P＜0.05). The frequencies of IL-8 (781C/T) CC, CT, TT genotypes in cases and controls were 38.61%,40.59%,20.79% and 46.28%,40.50%, 13.22%, respectively, there zwas no significant difference (P＞0.05). The frequencies of IL-8 (RA+860) GG, GC and CC genotypes in cases and controls were 75.25%, 21.78%, 2.97%, 80.17%, 14.88%, 4.96%, respectively, there was no significant difference (P＞0.05). Conclusions IL-8 (Met31 Arg and -251A/T) genetic polymorphisms might play a role in the development of silicosis. The risk of pneumoconiosis in workers carrying (Met31 Arg) genotype GT is likely to increase.The risk of pneumoconiosis in workers carrying IL-8 (-251A/T) AA genotype is likely to decrease. The relationship between IL-8 781C/T and RA+860 genes polymorphisms and silicosis is not found.
