CAJ | 학술논문

目的观察X射线照射后小鼠外周血网织红细胞微核(MN-RET)和骨髓嗜多染红细胞微核(MN-PCE)的变化,为探索快速、高通量的辐射生物剂量计奠定基础.方法 54只ICR雄性小鼠随机分为3组,每组18只,分别施予X射线全身照射,吸收剂量分别为0、0.5、1、2、4和5 Gy.在照射后24、48和72 h,分别采用流式细胞术(FCM)和人工镜检的方法,观察外周血MN-RET和骨髓MN-PCE的变化.结果在0.5～5.0 Gy范围内,3个时间点外周血MN-RET和骨髓MN-PCE均随剂量的增加而升高,剂量-效应曲线可拟合成直线回归方程(t=10.26～25.77,P<0.05);外周血MN-RET和骨髓MN-PCE之间明显相关(r=0.986-0.996,P<0.05).结论 FCM检测MN-RET有望成为快速、高通量的辐射生物剂量计或辅助诊断方法.
목적 관찰X사선조사후소서외주혈망직홍세포미핵(MN-RET)화골수기다염홍세포미핵(MN-PCE)적변화,위탐색쾌속、고통량적복사생물제량계전정기출.방법 54지ICR웅성소서수궤분위3조,매조18지,분별시여X사선전신조사,흡수제량분별위0、0.5、1、2、4화5 Gy.재조사후24、48화72 h,분별채용류식세포술(FCM)화인공경검적방법,관찰외주혈MN-RET화골수MN-PCE적변화.결과 재0.5～5.0 Gy범위내,3개시간점외주혈MN-RET화골수MN-PCE균수제량적증가이승고,제량-효응곡선가의합성직선회귀방정(t=10.26～25.77,P<0.05);외주혈MN-RET화골수MN-PCE지간명현상관(r=0.986-0.996,P<0.05).결론 FCM검측MN-RET유망성위쾌속、고통량적복사생물제량계혹보조진단방법.
objective To study the changes of reticulocyte micronueleus(MN-RET)from peripherai blood and polychromatic erythrocyte mieronucleUS(MN-PCE)from bone marrow in mice following exposure to X-rays in order to provide an experimental basis for exploring possible hish-throughput radiation biodosimeter.Methods Male ICR mice were whole-body irradiated with 0,0.5,1,2,4 and 5 Gy at a dose rate of 0.488 Gy/min.MN-RET from peripheral blood wag scored with FCM and MN-PCE from bone marrow was scored with manual microscopy at 24,48 and 72 h post-irradiation.Results Both MN-RET and MN-PCE rates increaged with doses in the range of 0-5 Gy at 24,48 and 72 h after WBI.The dose-response relationship can be fit with linear equations(t=10.26-25.77,P<0.05).The correlation coeffcients between MN-RET from peripheral blood and MN-PCE from bone mallow were highly significant(r=0.986-0.996,P<0.05).Conclusions In view of its simplicity,accuracy and high throughput capacity,FCM scoring of peripheral blood MN-RET may be a candidate for radiation biodosimetry,More work should be carried out on human specimens to investigate this possibility.