CAJ | 학술논문

目的观察依达拉奉(ED)对脓毒血症大鼠肝及心肌线粒体琥珀酸脱氢酶(SDH)的影响.方法 SD大鼠30只随机分为假手术组(A组)、手术对照组(B组)、ED治疗组(C组),B组和C组均予盲肠结扎穿刺法制作大鼠脓毒血症模型,三组术前15 min及术后3 h各皮下注射(sci)盐酸左氧氟沙星20 mg/kg,C组于术前15 min及术后3 h各sci ED 5 mg/kg,三组术后18 h取肝及心肌,测线粒体SDH活力,并对肝及心肌线粒体进行电镜检查.结果 B组肝及心肌线粒体SDH活力[(0.21±0.07)U/mgprot,(0.23±0.08)U/mgprot]显著低于A组[(0.33±0.10)U/mgprot,(0.38±0.12)U/mgprot];C组肝及心肌线粒体SDH活力[(0.31±0.08)U/mgprot,(0.36±0.11)U/mgprot]显著高于B组,B组电镜下可见肝及心肌线粒体轻度水肿,内质网扩张,C组心肌线粒体嵴多、致密.结论脓毒血症大鼠肝及心肌线粒体结构破坏,SDH活力下降;ED能有效地升高其线粒体SDH活力,保护线粒体结构和功能.
목적 관찰의체랍봉(ED)대농독혈증대서간급심기선립체호박산탈경매(SDH)적영향.방법 SD대서30지수궤분위가수술조(A조)、수술대조조(B조)、ED치료조(C조),B조화C조균여맹장결찰천자법제작대서농독혈증모형,삼조술전15 min급술후3 h각피하주사(sci)염산좌양불사성20 mg/kg,C조우술전15 min급술후3 h각sci ED 5 mg/kg,삼조술후18 h취간급심기,측선립체SDH활력,병대간급심기선립체진행전경검사.결과 B조간급심기선립체SDH활력[(0.21±0.07)U/mgprot,(0.23±0.08)U/mgprot]현저저우A조[(0.33±0.10)U/mgprot,(0.38±0.12)U/mgprot];C조간급심기선립체SDH활력[(0.31±0.08)U/mgprot,(0.36±0.11)U/mgprot]현저고우B조,B조전경하가견간급심기선립체경도수종,내질망확장,C조심기선립체척다、치밀.결론 농독혈증대서간급심기선립체결구파배,SDH활력하강;ED능유효지승고기선립체SDH활력,보호선립체결구화공능.
Objective To observe the effect on succinate dehydrogenase (SDH) of mitochondria in myocardium and liver in sepsis rats treated with edaravone. Methods 30 Sprague-Dawley rats were divided into 3 groups: sham operated group ( group A ), controlled operated group ( group B ), treated group with edaravone (group C). The model of sepsis rats was made by the way of caecum ligated and punctured and 20mg/kg lactate levofloxacin was subcutaneously injected (sci) 15min before and 3h after operation in three group. 5mg/kg edaravone were sci 15min before and 3h after operation in group C. Liver and myocardium were taken from all of them 18h after operation. The activities of SDH in myocardial and hepatic mitochondria were detected, pathological change of mitochondria in liver and myocardium were observed. Results The activities of SDH in myocardial and hepatic mitochondria in group B [ (0. 21 ± 0. 07 ) U/mgprot, (0. 23± 0. 08 ) U/mgprot ] were significantly decreased compared with group A [ ( 0. 33 ± 0. 10 ) U/mgprot, ( 0. 38±0. 12)U/mgprot]. The activities of those in group C[ (0.31 ±0. 08) U/mgprot, (0. 36 ±0. 11)U/mgprot] were significantly increased than group B. Myocardial and hepatic mitochondria swelling and endocytoplasmic reticulum expanding were found in group B by electron microscope, while it showed normal in group C. Conclusion Hepatic and myocardial mitochondrial structure were destroyed and activities of SDH were decreased in sepsis rats. They could be effectively protected by edaravone.