CAJ | 학술논문

目的了解河北省肾综合征出血热(HFRS)疫源地宿主动物所携带汉坦病毒的基因型和基因亚型.方法根据76-118株和R22株的保守序列设计型特异性引物,用RT-nested PCR从IFA筛选的阳性鼠肺中扩增部分M片段,经琼脂糖凝胶电泳进行基因分型,并从中选取部分扩增产物纯化回收后进行核苷酸序列测定,用DNAStar软件包进行基因分析.结果 32份经IFA筛选阳性的鼠肺标本经RT-nested PCR扩增出418bp特异性目的片段,均为SEO型.10份扩增产物测序结果表明G2片段变异不大,同源性高达98.0%～100%,与R22株和76-118株的同源性分别为93.3%～94.3%和67.7%～69.0%.结论依据G2片段,河北省HFRS疫源地汉坦病毒基因型以SEO型病毒为主,主要为S3亚型,同亚型病毒基因高度同源,遗传物质相对稳定.不同鼠种可携带同亚型汉坦病毒.
목적 료해하북성신종합정출혈열(HFRS)역원지숙주동물소휴대한탄병독적기인형화기인아형.방법 근거76-118주화R22주적보수서렬설계형특이성인물,용RT-nested PCR종IFA사선적양성서폐중확증부분M편단,경경지당응효전영진행기인분형,병종중선취부분확증산물순화회수후진행핵감산서렬측정,용DNAStar연건포진행기인분석.결과 32빈경IFA사선양성적서폐표본경RT-nested PCR확증출418bp특이성목적 편단,균위SEO형.10빈확증산물측서결과 표명G2편단변이불대,동원성고체98.0%～100%,여R22주화76-118주적동원성분별위93.3%～94.3%화67.7%～69.0%.결론 의거G2편단,하북성HFRS역원지한탄병독기인형이SEO형병독위주,주요위S3아형,동아형병독기인고도동원,유전물질상대은정.불동서충가휴대동아형한탄병독.
Objective To know the genotype and subtype of hantavims (HV) carried by host animals in HFRS natural epidemic area of Hebei province. Methods According to the conservative sequence of 76-118 and R22 strains, specific primers were designed. RT-nested PCR was used to amplify partial M segments from the positive rat lungs screened by IFA. Agarose gel electrophoresis was used to identify the types. Nucleotides were assayed from partial products after purification and reclaim. Gene analysis was carried on with DNAStar package.Results 32 specimens, which were positive screened by IFA, were amplified the specific segment (418bp) and all belonged to type SEO. Sequencing results of 10 partial segments indicated that G2 segment had little variability and nucleotide homology reached to 98.0%-100.0%. Comparing with the R22 and 76-118 strains, homology was 93.3%-94.3% and 67.7%-69.0% respectively. Conclusion According to G2 segment, SEO was the major type in Hebei HFRS natural epidemic area and S3 was the major subtype. HV which belonged to the same subtype had high homology and genetic materials were correspondingly stable. Different rats could carry the same subtype of HV.