中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
7期
660-664
,共5页
米利古%王远志%李永祥%艾尔肯江·孜帕尔%曹红艳%吴长东%陈伟%袁俐
米利古%王遠誌%李永祥%艾爾肯江·孜帕爾%曹紅豔%吳長東%陳偉%袁俐
미리고%왕원지%리영상%애이긍강·자파이%조홍염%오장동%진위%원리
结核分枝杆菌%维吾尔族%多位点可变串联重复序列分析%基因分型
結覈分枝桿菌%維吾爾族%多位點可變串聯重複序列分析%基因分型
결핵분지간균%유오이족%다위점가변천련중복서렬분석%기인분형
Mycobacterium tuberculosis%Uygur%Multiple locus variable numbers of tandem repeats%Genotyping
目的 应用多位点数目可变串联重复序列(VNTR)分析技术,对新疆南疆地区维吾尔族结核病患者结核分枝杆菌临床分离株进行基因分型,探讨其数目VNTR基因型种类及其分布.方法 收集结核分枝杆菌,采用PCR和琼脂糖凝胶电泳技术,结合BioNumerics 5.0软件,对其24个VNTR位点进行结果分析.结果 分离出151株结核分枝杆菌,分为8个基因群151个基因型,其中Ⅵ群为主要基因群,占44.4%,有67个基因型,其次是Ⅷ群(23.2%)和Ⅳ群(20.5%).结论 新疆南疆地区维吾尔族结核病患者的结核分枝杆菌存在明显基因多态性,且存在主要流行菌群.
目的 應用多位點數目可變串聯重複序列(VNTR)分析技術,對新疆南疆地區維吾爾族結覈病患者結覈分枝桿菌臨床分離株進行基因分型,探討其數目VNTR基因型種類及其分佈.方法 收集結覈分枝桿菌,採用PCR和瓊脂糖凝膠電泳技術,結閤BioNumerics 5.0軟件,對其24箇VNTR位點進行結果分析.結果 分離齣151株結覈分枝桿菌,分為8箇基因群151箇基因型,其中Ⅵ群為主要基因群,佔44.4%,有67箇基因型,其次是Ⅷ群(23.2%)和Ⅳ群(20.5%).結論 新疆南疆地區維吾爾族結覈病患者的結覈分枝桿菌存在明顯基因多態性,且存在主要流行菌群.
목적 응용다위점수목가변천련중복서렬(VNTR)분석기술,대신강남강지구유오이족결핵병환자결핵분지간균림상분리주진행기인분형,탐토기수목VNTR기인형충류급기분포.방법 수집결핵분지간균,채용PCR화경지당응효전영기술,결합BioNumerics 5.0연건,대기24개VNTR위점진행결과분석.결과 분리출151주결핵분지간균,분위8개기인군151개기인형,기중Ⅵ군위주요기인군,점44.4%,유67개기인형,기차시Ⅷ군(23.2%)화Ⅳ군(20.5%).결론 신강남강지구유오이족결핵병환자적결핵분지간균존재명현기인다태성,차존재주요류행균군.
Objective To investigate the application of the multiple locus variable numbers of tandem repeats(MLVA) in genotype Mycobacterium tuberculosis(MTB) strains isolated from Uygur in south of Xinjiang, and to understand the characteristics of genotype and distribution. Methods One hundred and fifty-one Mycobacterium tuberculosis strains were collected from Uygur in south of Xinjiang which contains three regions, Kashgar, Hotan and Kizilsu kirghiz. Twenty-four tandem repeats loci in the total genome of MTB were analyzed by PCR and agarose gel electrophoresis method. The characteristics on polymorphism of DNA fingerprinting of 151 MTB strains were analyzed with BioNumerics 5.0 software. Results Twenty-four MLVA loci of 151 MTB strains were analyzed respectively. The results showed that there were obvious polymorphisms of VNTRs. The clustering of genotype showed that these strains could be categorized into 8 gene groups( Ⅰ , Ⅱ , Ⅲ ,Ⅳ, Ⅴ ,Ⅵ,Ⅶ ,Ⅷ) and 151 genotypes. Sixty-seven isolates(44.4% ) belonged to group Ⅵ. 23.2% were group Ⅷ including 35 genotypes, 20. 5% were group Ⅳ including 31 genotypes. The group Ⅵ prevailed mostly in the Kashgar. The group Ⅲ prevailed mostly in the HOTAN. Conclusion The results showed there were obvious polymorphisms of VNTRs of Mycobacterium tuberculosis clinical strains preliminarily. Group Ⅵ was the predominant prevalent strain in south of Xinjiang.