CAJ | 학술논문

目的检测糖皮质激素受体-α、p(GR-α、GR-β)在翼状胬肉组织中的表达,探讨其在翼状胬肉发病机制中的作用.方法取39例翼状胬肉组织(其中初发30例,复发9例),应用荧光定量逆转录PCR和免疫组化技术检测GR-α、βmRNA和蛋白表达,并选择8例正常人的球结膜做为对照.结果翼状胬肉及正常人的球结膜组织中均存在GR-αmRNA表达,但GR-β mRNA表达均为阴性.其中39例翼状胬肉中的GR-α mRNA为(10.40±1.42)× 10<'3>拷贝/μg,8例正常眼球结膜中GR-α mRNA为(2.23±0.32)×10<'3>拷贝/μg,差异有统计学意义(t=4.82,P<0.01).其中初发性胬内(30例)表达为(10.40±0.40)×10<'3>拷贝/μg,而复发性胬肉(9例)为(10.50±0.30)×10<'3>拷贝/μg,较初发性胬内略高,但差异无统计学意义(t=0.55,P>0.05).结论翼状胬内组织中GR-αmRNA及其蛋白表达明显增高,这可能是翼状胬肉发生、发展的重要原因之一,但其作用机制尚待于进一步研究.
목적 검측당피질격소수체-α、p(GR-α、GR-β)재익상노육조직중적표체,탐토기재익상노육발병궤제중적작용.방법 취39례익상노육조직(기중초발30례,복발9례),응용형광정량역전록PCR화면역조화기술검측GR-α、βmRNA화단백표체,병선택8례정상인적구결막주위대조.결과 익상노육급정상인적구결막조직중균존재GR-αmRNA표체,단GR-β mRNA표체균위음성.기중39례익상노육중적GR-α mRNA위(10.40±1.42)× 10<'3>고패/μg,8례정상안구결막중GR-α mRNA위(2.23±0.32)×10<'3>고패/μg,차이유통계학의의(t=4.82,P<0.01).기중초발성노내(30례)표체위(10.40±0.40)×10<'3>고패/μg,이복발성노육(9례)위(10.50±0.30)×10<'3>고패/μg,교초발성노내략고,단차이무통계학의의(t=0.55,P>0.05).결론 익상노내조직중GR-αmRNA급기단백표체명현증고,저가능시익상노육발생、발전적중요원인지일,단기작용궤제상대우진일보연구.
Objective To investigate whether theglucocorticoid receptor would be expressed in pterygium and dis-cuss its significant. Methods GR- α and GR-β mRNA expression were detected by a real -time fluorescent quantitative RT- PCR. GR-α and GR-β protein expression were examined by Western- blotting analysis and immunohistochemicaltechnique from 39 patients with pterygium (30 primary pterygium, 9 recurrent pterygium). Normal conjunctivae(n =8) were as controls. Results The expression of GR -α nLRNA were (10. 40 ± 1. 42) × 10<'3>GR -α cDNA copies/μg total RNA in pterygium and (2.23 ±0. 32) × 10<'3>GR- α cDNA copies/μg total RNA in normal conjunctivae. The expression of GR- β mRNA was not detected in pterygium and in normal conjunctivae. There was no difference between the expression of GR -α mRNA in recurrent pterygium(10.50 ± 0.30)and the primary pterygium(10. 40 ± 0. 40)(P > 0. 05). There was significantly difference between the expression of GR - α mRNA in pterygium and the normal conjunctivae. Western - blotting analysis and immunohistochemical technique confirmed these findings for the protein transcripts of these genes. Conclusions These results demonstrate that the expression of GR-α mRNA and protein are higher in pterygium and probably are one of the important reasons. But its effection and meehanismin pterygium still need more investigate.