CAJ | 학술논문

利用RT_PCR方法从拟南芥(Arabidopsis thaliana (L.) Heynh.)中克隆了花分生组织决定基因(flower_meristem_identity gene)AP1，进行了全序列测定。测序结果显示，所得到的基因与发表的序列仅有一个碱基的差异，但并不影响蛋白质的一级结构。将AP1基因克隆入植物中间载体p208，通过根癌土壤杆菌(Agrobacterium tumefaciens (Smith et Townsend) Conn)介导的方法转化矮牵牛(Petunia hybrida Vilm.)。对转基因植株进行了PCR和Southern检测，所得到的两个株系的转基因矮牵牛在R0代即表现出提前且持续不断地开花的特性，与对照差异显著。
이용RT_PCR방법종의남개(Arabidopsis thaliana (L.) Heynh.)중극륭료화분생조직결정기인(flower_meristem_identity gene)AP1，진행료전서렬측정。측서결과현시，소득도적기인여발표적서렬부유일개감기적차이，단병불영향단백질적일급결구。장AP1기인극륭입식물중간재체p208，통과근암토양간균(Agrobacterium tumefaciens (Smith et Townsend) Conn)개도적방법전화왜견우(Petunia hybrida Vilm.)。대전기인식주진행료PCR화Southern검측，소득도적량개주계적전기인왜견우재R0대즉표현출제전차지속불단지개화적특성，여대조차이현저。
Flower_meristem_identity gene AP1 was isolated from Arabidopsis thaliana (L.) Heynh. by RT_PCR. The result of the sequencing showed that only a single nucleotide change was found in this AP1 gene compared with the published sequence, but this changed nucleotide did not effect the primary structure of the protein. Driven by the CaMV 35S promoter, AP1 was stably integrated into the genome of Petunia hybrida Vilm. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated transformation. The intergration of the transgene was identified by PCR and Southern blot analysis. The R0 generation of two transgenic lines could flower earlier and constantly even in the tissue culture flask, differing from the non_transformed Petunia hybrida.