CAJ | 학술논문

目的观察小胶质细胞(BV2)在骨髓基质细胞(MSC)接受受损的PC12上清刺激后,对其神经营养功能的影响.方法采用原代细胞培养法培养骨髓基质细胞(MSC),以 BV2和PC12细胞株分别代表小胶质细胞和神经细胞进行传代培养,流式细胞术检测PC12凋亡,ELISA检测培养上清中的神经营养因子.结果小胶质细胞(BV2)和损伤PC12上清同时刺激MSC时,后者分泌的上清能显著降低受损PC12的凋亡数目.结论小胶质细胞的存在有利于接受受损神经细胞上清刺激的MSC发挥神经营养及神经支持功能.
목적관찰소효질세포(BV2)재골수기질세포(MSC)접수수손적PC12상청자격후,대기신경영양공능적영향.방법채용원대세포배양법배양골수기질세포(MSC),이 BV2화PC12세포주분별대표소효질세포화신경세포진행전대배양,류식세포술검측PC12조망,ELISA검측배양상청중적신경영양인자.결과소효질세포(BV2)화손상PC12상청동시자격MSC시,후자분비적상청능현저강저수손PC12적조망수목.결론소효질세포적존재유리우접수수손신경세포상청자격적MSC발휘신경영양급신경지지공능.
[Objective] To study whether and how microglias (BV2) affect the neurotrophic acitvity of marrow stromal cells (MSC) after being stimulated with injured PC12 media. [Methods] Primary culture was adopted for MSC culture, BV2 and PC12 were cultured as substitutes for microglias and neurons, respectively. Flow Cytometry was used to detect PC12 apoptosis, and ELISA for neurotrophic factor assay in MSC supernatant. [Results] With BV coculture, the PC12 apoptosis was reduced to the least when incubated with injured PC12 media. [Conclusion]Microglias are helpful in the neurotrophic activity of MSC stimulated with supernatant of injured neurons.