CAJ | 학술논문

背景:急性呼吸窘迫综合征重要病理改变是肺泡一毛细血管膜屏障的损伤,以及所导致肺泡渗出液中富含蛋白质的肺水肿.骨髓间充质干细胞能够继续分化和不断自我更新,并最终分化为多种类型细胞,这有可能为治疗肺损伤提供一个新的途径.目的:探讨骨髓间充质干细胞移植对内毒素诱发急性肺损伤模型兔的保护作用.设计、时间及地点:随机对照动物实验,于2007-10/2008-01在唐都医院中心实验室完成.材料:家兔20只,2只用于制备骨髓间充质干细胞,剩余18只随机分为3组:盐水对照组、肺损伤模型组、细胞移植组,6只,组.内毒素为Sigma公司产品.方法:Ficoll法分离培养兔骨髓间充质干细胞,传至第3代备用.肺损伤模型组、细胞移植组兔通过向气管内滴注内毒素建立急性肺损伤,急性呼吸窘迫综合征模型,造模成功30 min后,细胞移植组经右侧颈静脉注入骨髓间充质干细胞悬浮液2 mL(细胞数1×105),盐水对照组、肺损伤模型组同法给予等量生理盐水.主要观察指标:支气管肺泡灌洗液中性粒细胞数日、肺组织湿干比及蛋白含量,肺组织病理变化.结果:湿十比值升高提示肺水肿的存在,中性粒细胞数量增加提示较重的炎性反应存在,蛋白含量升高提示肺泡一毛细血管内膜屏障受损.移植48 h后与盐水对照组比较,肺损伤模型组支气管肺泡灌洗液中性粒细胞数目、蛋白含量均明显降低(P<0.01),湿干比明显升高(P<0.01);与肺损伤模型组比较,细胞移植组支气管肺泡灌洗液中性粒细胞数目、蛋白含量均明显升高(P<0.01),湿干比明显降低(P<0.01).苏木精一伊红染色结果显示,盐水对照组肺泡组织结构正常,肺损伤模型组肺组织出现典型的急性肺损伤改变,细胞移植组肺组织病理变化较轻.结论:利用骨髓间充质干细胞移植可显著减轻内毒素诱导的急性肺损伤.
배경:급성호흡군박종합정중요병리개변시폐포일모세혈관막병장적손상,이급소도치폐포삼출액중부함단백질적폐수종.골수간충질간세포능구계속분화화불단자아경신,병최종분화위다충류형세포,저유가능위치료폐손상제공일개신적도경.목적:탐토골수간충질간세포이식대내독소유발급성폐손상모형토적보호작용.설계、시간급지점:수궤대조동물실험,우2007-10/2008-01재당도의원중심실험실완성.재료:가토20지,2지용우제비골수간충질간세포,잉여18지수궤분위3조:염수대조조、폐손상모형조、세포이식조,6지,조.내독소위Sigma공사산품.방법:Ficoll법분리배양토골수간충질간세포,전지제3대비용.폐손상모형조、세포이식조토통과향기관내적주내독소건립급성폐손상,급성호흡군박종합정모형,조모성공30 min후,세포이식조경우측경정맥주입골수간충질간세포현부액2 mL(세포수1×105),염수대조조、폐손상모형조동법급여등량생리염수.주요관찰지표:지기관폐포관세액중성립세포수일、폐조직습간비급단백함량,폐조직병리변화.결과:습십비치승고제시폐수종적존재,중성립세포수량증가제시교중적염성반응존재,단백함량승고제시폐포일모세혈관내막병장수손.이식48 h후여염수대조조비교,폐손상모형조지기관폐포관세액중성립세포수목、단백함량균명현강저(P<0.01),습간비명현승고(P<0.01);여폐손상모형조비교,세포이식조지기관폐포관세액중성립세포수목、단백함량균명현승고(P<0.01),습간비명현강저(P<0.01).소목정일이홍염색결과현시,염수대조조폐포조직결구정상,폐손상모형조폐조직출현전형적급성폐손상개변,세포이식조폐조직병리변화교경.결론:이용골수간충질간세포이식가현저감경내독소유도적급성폐손상.
BACKGROUND: The important pathological changes of acute respiratory distress syndrome (ARDS) is disruption of the lung alveolar-capillary membrane barrier and resultant pulmonary edema associated with a proteinaceous alveolar exudate. Bone marrow mesenchymal stem cells (BMSCs) are able to carry on dividing and renewing themselves, and can eventually develop into many other types of cells. This provides a new treatment for treating injury of lungs.OBJECTIVE: To investigate the prevention of endotoxin-induced acute lung injury in rabbit by BMSCs.DESIGN, TIME AND SETTING: The randomized controlled animal study was performed at the Central Laboratory of Tangdu Hospital from October 2007 to January 2008.MATERIALS: A total of 20 rabbits were used in this study. Two rabbits were utilized to culture BMSCs. Eighteen rabbits were randomly assigned to three groups, saline control group, acute lung injury group and cell transplantation group (n = 6). Endotoxin was purchased from Sigma, USA.METHODS: Rabbit BMSCs were isolated and cultured by the Ficoll method. At the third passage, BMSCs were harvested for use.In the acute lung injury and call transplantation groups, endotoxin was infused into the trachea to establish models of acute lung injury/ARDS. Thirty minutes following model establishment, 2 mL BMSC suspension (1 x 105) was infused into the right jugular vein in the cell transplantation group. An equal volume of saline was injected into the saline control and acute lung injury groups.MAIN OUTCOME MEASURES: Number of neutrophilic granulocyte, wet to dry weight ratio of lung tissue, protein content and pathological changes in lung tissue in bronchoalveolar lavage fluid were measured.RESULTS: The increase in wet to dry weight ratio indicated the existence of pulmonary edema. The increase in neutrophilic granulocyte number suggested severe inflammatory reaction. The increased protein content showed the damage to lung alveolar-capillary membrane barrier. Following 48 hours of transplantation, neutrophilic granulocyte number and protein content in bronchoalveolar lavage fluid was significantly decreased (P < 0.01), and wet to dry weight ratio was significantly increased (P < 0.01) in the acute lung injury group compared with the saline control group. Compared with the acute lung injury group,neutrophilic granulocyte number and protein content was significantly increased (P < 0.01), and wet to dry weight ratio was significantly diminished (P < 0.01) in bronchoalveolar lavage fluid in the call transplantation group. Hematoxylin-eosin staining suggested that pulmonary alveoli was normal in the saline control group, presented typical acute lung injury in the acute lung injury group, and the pathological changes were mild in the cell transplantation group.CONCLUSION: BMSC transplantation can significantly reduce endotoxin-induced acute lung injury.